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234109
Sigma-AldrichColcemid - CAS 477-30-5 - Calbiochem
Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.
Catalogue Number
234109
Brand Family
Calbiochem®
Synonyms
Demecolcine
References
References
Urbani, L., et al. 1995. Exp. Cell Res. 219, 159. Kanda, R., et al. 1994. J. Radiat. Res. 35, 41. Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373. Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214. Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.
Depolymerizes microtubules and limits microtubule formation.
Purity
≥98% by HPLC
Physicochemical Information
Cell permeable
N
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
GH0800000
Safety Information
R Phrase
R: 25-63
Toxic if swallowed. Possible risk of harm to the unborn child.
S Phrase
S: 22-36/37/39-45
Do not breathe dust. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+15°C to +30°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.
Colcemid - CAS 477-30-5 - Calbiochem Certificates of Analysis
Title
Lot Number
234109
References
Reference overview
Urbani, L., et al. 1995. Exp. Cell Res. 219, 159. Kanda, R., et al. 1994. J. Radiat. Res. 35, 41. Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373. Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214. Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
03-April-2008 RFH
Synonyms
Demecolcine
Description
Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.
Form
Crystalline powder
CAS number
477-30-5
RTECS
GH0800000
Chemical formula
C₂₁H₂₅NO₅
Structure formula
Purity
≥98% by HPLC
Solubility
Ethanol and chloroform (10 mg/ml)
Storage
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Toxic & Carcinogenic / Teratogenic
Merck USA index
14, 2887
References
Urbani, L., et al. 1995. Exp. Cell Res. 219, 159. Kanda, R., et al. 1994. J. Radiat. Res. 35, 41. Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373. Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214. Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.
