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204897 Complement C4b, Human

Complement C4b, Human, is a native, 193 kDa glycoprotein composed of the modified C4 α-chain (α') and intact β- and γ-chains complement component.

Complement C4b, Human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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204897
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
204897-250UG Plastic ampoule 250 μg
Description
OverviewNative, human C4b complement component. Cleavage of the C4 α-chain at peptide bond 77 by activated C1 enzyme results in the production of C4a (M.W. 8,758) and C4b (M.W. 193,000) fragments. The C4b fragment is a glycoprotein composed of the modified C4 α-chain (α′) and intact β- and γ-chains. Like C3b, C4b has the transient ability to form a covalent ester bond with a variety of target cell surfaces. Once bound to the target surface, C4b becomes an essential non-enzymatic subunit of the classical pathway C3 cleaving enzyme (C4b,C2a). In addition, surface bound C4b has opsonic and immune adherence activities which are mediated via binding to the CR1 (CD35) complement receptor which is found on a variety of inflammatory cells.
Catalogue Number204897
Brand Family Calbiochem®
References
ReferencesWeisman, H.F., et al. 1990. Science 249, 146.
Holers, M.V., et al. 1985. Immunology Today 6, 188.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
SourcePrepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsIgG, IgA, IgM, Factor B, Factor H, C3, ceruloplasmin, or albumin: ≤trace amounts
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
204897-250UG 04055977219852

Documentation

Complement C4b, Human SDS

Title

Safety Data Sheet (SDS) 

Complement C4b, Human Certificates of Analysis

TitleLot Number
204897

References

Reference overview
Weisman, H.F., et al. 1990. Science 249, 146.
Holers, M.V., et al. 1985. Immunology Today 6, 188.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-May-2008 RFH
DescriptionNative, human C4b complement component. Cleavage of the C4 α-chain at peptide bond 77 by activated C1 enzyme results in the production of C4a (M.W. 8,758) and C4b (M.W. 193,000) fragments. The C4b fragment is a glycoprotein composed of the modified C4 α-chain (α') and intact β- and γ-chains. Like C3b, C4b has the transient ability to form a covalent ester bond with a variety of target cell surfaces. Once bound to the target surface, C4b becomes an essential non-enzymatic subunit of the classical pathway C3 cleaving enzyme (C4b,C2a). In addition, surface bound C4b has opsonic and immune adherence activities, which are mediated via binding to the CR1 (CD35) complement receptor, which is found on a variety of inflammatory cells.
FormLiquid
FormulationIn PBS, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
SourcePrepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Purity≥95% by SDS-PAGE
ContaminantsIgG, IgA, IgM, Factor B, Factor H, C3, ceruloplasmin, or albumin: ≤trace amounts
PreservativeNone
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesWeisman, H.F., et al. 1990. Science 249, 146.
Holers, M.V., et al. 1985. Immunology Today 6, 188.