Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

S7804 CpG WIZ® E-Cadherin Amplification Kit

The CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP).

View Products on Sigmaaldrich.com
S7804
25 assays  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberS7804
Brand Family Chemicon®
Trade Name
  • CpG Wiz
  • Chemicon
DescriptionCpG WIZ® E-Cadherin Amplification Kit
OverviewE-cadherin is a cell adhesion protein that suppresses tumor cell invasion and metastasis in tumor models. On mechanism regulating the expression of the E-cadherin gene is methylation of the promoter region. The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated) for E-cadherin are also included.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
Alternate Names
  • CD324
References
Product Information
HS Code3822 19 90
Quality LevelMQ100
Applications
ApplicationThe CpG WIZ E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP).
Key Applications
  • PCR
Application NotesPrinciples of the Technique

Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® E-cadherin Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
Biological Information
Species Reactivity
  • Human
Entrez Gene Number
Entrez Gene SummaryThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
Gene Symbol
  • CDH1
  • CDHE
  • E-cadherin
  • Arc-1
  • Uvomorulin
  • UVO
  • Cadherin-1
  • CD324
  • uvomorulin
  • ECAD
  • LCAM
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P12830 # E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
SIZE: 882 amino acids; 97456 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLG7.
SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm.
TISSUE SPECIFICITY: Non-neural epithelial tissues.
PTM: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.
DISEASE: "SwissProt: P12830 # Defects in CDH1 are involved in dysfunction of the cell- cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. & Defects in CDH1 are a cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. & Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089]."
SIMILARITY: SwissProt: P12830 ## Contains 5 cadherin domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size25 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
S7804 04053252667114

Documentation