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S8005 CpGenome™ 5-mC and 5-hmC DNA Standard Set

This set can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.

CpGenome™ 5-mC and 5-hmC DNA Standard Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
S8005
2 µg per vial  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberS8005
Brand Family Upstate
Trade Name
  • CpGenome
  • Upstate
DescriptionCpGenome™ 5-mC and 5-hmC DNA Standard Set
OverviewCpGenome™ 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications.

Materials Provided:

Three vials, each containing 2 µg (40 µL) of CpGenome™ Cytosine DNA Standard, CpGenome™ 5-mC DNA Standard, or CpGenome™ 5-hmC DNA Standard, at a concentration of 50 ng/μL.

Validation:

The three Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

CpGenome is a trademark of Serologicals Corporation.
References
Product Information
PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Quality LevelMQ100
Applications
ApplicationThis set can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.
Biological Information
Species Reactivity
  • Human
Modifications
  • Methylation
  • Hydroxylation
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended Storage: Stable for 6 months at -20°C from date of receipt.
Packaging Information
Material Size2 µg per vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
S8005 04053252648427

Documentation

CpGenome™ 5-mC and 5-hmC DNA Standard Set SDS

Title

Safety Data Sheet (SDS) 

CpGenome™ 5-mC and 5-hmC DNA Standard Set Certificates of Analysis

TitleLot Number
CpGenome 5-mC and 5-hmC DNA Standard Set - Q2023994 Q2023994
CpGenome™ 5-mC and 5-hmC DNA Standard Set 3085274
CpGenome™ 5-mC and 5-hmC DNA Standard Set - 3248118 3248118
CpGenome™ 5-mC and 5-hmC DNA Standard Set - 3505979 3505979
CpGenome™ 5-mC and 5-hmC DNA Standard Set - 3783309 3783309
CpGenome™ 5-mC and 5-hmC DNA Standard Set - 4082426 4082426
CpGenome™ 5-mC and 5-hmC DNA Standard Set -2532354 2532354
CpGenome™ 5-mC and 5-hmC DNA Standard Set - 2268805 2268805

Brochure

Title
Shaping Epigenetics Discovery - DNA Methylation Brochure

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics