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23-031 Cyclin E1 complex

Cyclin E1 complex MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
23-031
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman Substrate
Description
Catalogue Number23-031
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCyclin E1 complex
OverviewN-terminal c-Myc, 6His-tagged, recombinant human Smad7 full length, expressed by baculovirus in Sf21 insect cells.
Alternate Names
  • CDK2/CyclinE
  • CDK2/Cyclin E1
Background InformationCyclin E1 functions as a regulator of the cell cycle, promoting the G1 to S phase transition by binding to and activating the cyclin dependent kinase CDK2. The abundance of cyclin E1 fluctuates during a normal cell cycle and is controlled at the level of gene transcription and by ubiquitin dependent proteolysis. Non-complexed cyclin E1 is degraded by the Cul3 pathway, however when cyclin E1 is bound to CDK2 its ubiqutination is dependent on a positive phosphorylation status, which enables its recognition by SCFFbw7. The impact of cyclin E1 quantities on cell cycle regulation is reflected in the elevated levels expressed by many types of human tumours.
References
Product Information
Quality LevelMQ100
Applications
Key Applications
  • Enzyme Assays
Biological Information
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade ComponentSubstrate
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
23-031 04053252294761

Related Products & Applications

Alternative Packsize

Catalog Number Description
23-031M Cyclin E1 Protein complex, 250 µg

Product Families

Ubiquitin Proteasome System: Enzymes and Substrates

Millipore has removed the complexity of Ubiquitination by providing functionally validated enzymes and substrates, making the pathway readily accessible for research and drug discovery.
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Categories

Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitination Substrates
Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Ubiquitination Substrates