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239803 Cyclopamine, V. californicum - CAS 4449-51-8 - Calbiochem

Cyclopamine, V. californicum, CAS 4449-51-8, is a cell-permeable steroidal alkaloid & cholesterol mimic that specifically antagonizes Sonic Hedgehog signaling through direct interaction with Smo.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Shh Signaling Antagonist I

Primary Target: Hh signaling in Shh-light2 assay
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
4449-51-8C₂₇H₄₁NO₂

Products

Catalog NumberPackaging Qty/Pack
239803-1MG Glass bottle 1 mg
239803-5MG Glass bottle 5 mg
Description
OverviewA cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. Also available as a 10 mM solution in Ethanol (Cat. No. 239806).
Catalogue Number239803
Brand Family Calbiochem®
SynonymsShh Signaling Antagonist I
References
ReferencesBerman, D.M., et al. 2003. Nature, 425, 846.
Thayer, S.P., et al. 2003. Nature, 425, 851.
Watkins, D.N., et al. 2003. Nature 422, 313.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
Berman, D.M., et al. 2002. Science 297, 1559.
Taipale, J., et al. 2000. Nature 406, 1005.
Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA 95, 13036.
Cooper, M.K., et al. 1998. Science 280, 1603.
Incardona, J.P., et al. 1998. Development 125, 3553.
Product Information
CAS number4449-51-8
ATP CompetitiveN
FormOff-white solid
Hill FormulaC₂₇H₄₁NO₂
Chemical formulaC₂₇H₄₁NO₂
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetHh signaling in Shh-light2 assay
Primary Target IC<sub>50</sub>20 nM
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSGY0750000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Carcinogenic / Teratogenic
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
239803-1MG 04055977198867
239803-5MG 04055977198874

Documentation

Cyclopamine, V. californicum - CAS 4449-51-8 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Cyclopamine, V. californicum - CAS 4449-51-8 - Calbiochem Certificates of Analysis

TitleLot Number
239803

References

Reference overview
Berman, D.M., et al. 2003. Nature, 425, 846.
Thayer, S.P., et al. 2003. Nature, 425, 851.
Watkins, D.N., et al. 2003. Nature 422, 313.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
Berman, D.M., et al. 2002. Science 297, 1559.
Taipale, J., et al. 2000. Nature 406, 1005.
Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA 95, 13036.
Cooper, M.K., et al. 1998. Science 280, 1603.
Incardona, J.P., et al. 1998. Development 125, 3553.

Brochure

Title
Biologics 33.2
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-October-2007 JSW
SynonymsShh Signaling Antagonist I
DescriptionA cell-permeable, steroidal alkaloid and cholesterol mimic that displays both teratogenic and anti-tumor properties. Disrupts cholesterol biosynthesis and specifically antagonizes shh (Sonic Hedgehog) signaling through direct interaction with Smo (smoothened), a protein that is related to G-protein-coupled receptors. Useful for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control.
FormOff-white solid
Intert gas (Yes/No) Packaged under inert gas
CAS number4449-51-8
RTECSGY0750000
Chemical formulaC₂₇H₄₁NO₂
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (4 mg/ml), Ethanol (5 mg/ml), or DMF (10 mg/ml). Ethanol stock solutions may require warming to achieve complete solubilization.
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Carcinogenic / Teratogenic
ReferencesBerman, D.M., et al. 2003. Nature, 425, 846.
Thayer, S.P., et al. 2003. Nature, 425, 851.
Watkins, D.N., et al. 2003. Nature 422, 313.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
Berman, D.M., et al. 2002. Science 297, 1559.
Taipale, J., et al. 2000. Nature 406, 1005.
Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA 95, 13036.
Cooper, M.K., et al. 1998. Science 280, 1603.
Incardona, J.P., et al. 1998. Development 125, 3553.