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71510 D-Tube™ Dialyzer Maxi, MWCO 12-14 kDa

D-Tube™ Dialyzer Maxi, MWCO 12-14 kDa MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71510
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
71510-3 Glass bottle 1 kit
Description
Overview
D-Tube™ Dialyzers Mini, Midi, Maxi, and Mega
The D-Tube™ Dialyzers be used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuge, or laborious steps to manipulate small sample volumes. The sample is added and removed using a standard laboratory pipet. Available with molecular weight cutoffs (MWCO) from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in four volume capacities: mini (10-250 µl), midi (50-800 micro;l), maxi (100-3000 µl), and mega (10ml, 15ml, and 20 ml). The membrane is ultra-clean, EDTA-treated regenerated cellulose, sulfur- and heavy metal-free.

The D-Tube Dialyzers Mini, Midi, and Maxi contain 10 D-Tube Dialyzers and one floating rack that can hold up to four D-Tube Dialyzers. The D-Tube Dialyzers Mega contain 10 or 50 tubes and one or 5 floating racks to hold one D-Tube Mega in the exchange buffer.

D-Tube™ Electroelution Accessory Kit
The D-Tube Electroelution Accessory Kit provides a support tray for the D-Tube Dialyzers (compatible with most commercially-available horizontal electrophoresis units) and optimized reagents for protein and nucleic acid precipitation following electroelution. The kit also contains an optimized buffer for the removal of SDS from extracted protein samples, making them compatible with MALDI-MS analysis. The combination of D-Tube Dialyzers and D-Tube Electroelution Accessory Kit provides a unique tool for extraction of any protein, protein-protein and protein-DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels with 60% recovery yield in less than 2 hours. The procedure efficiently recovers proteins and simultaneously removes ampholytes from proteins run on 2D gels. Extracted proteins are compatible with most downstream applications such as MALDIMS, animal immunization for antibody production, HPLC analysis, peptide mapping, and functional assays. In addition, D-Tube Dialyzers can be used for oligonucleotides, RNA and DNA extraction from both polyacrylamide and agarose gels. Efficient extraction (>90%) is achieved for 15 nt oligos and for up to 80 kbp DNA fragments.
Catalogue Number71510
Brand Family Novagen®
Features and benefits
  • Easy-to-handle dialyzers for buffer exchange and removal of urea, detergents
  • Ideal for electroelution of proteins, protein-DNA complexes, oligonucleotides, DNA, and RNA from polyacrylamide and agarose gels
  • One-step procedure that does not require syringes or any special equipment
  • Sample volume recovery > 97%
  • Protease, RNAse, DNAse, and PCR product free
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
71510-3 07790788053345

Documentation

D-Tube™ Dialyzer Maxi, MWCO 12-14 kDa SDS

Title

Safety Data Sheet (SDS) 

D-Tube™ Dialyzer Maxi, MWCO 12-14 kDa Certificates of Analysis

TitleLot Number
71510

Brochure

Title
Fast and Easy Sample Dialysis.
Protein Purification and Detection Tools

Citations

Title
  • Job E. Lopez, et al. (2007) Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine. Infection and Immunity 75, 2333-2342.
    		•

    User Protocols

    Title
    TB422 D-Tube™ Dialyzers: Mini, Midi, Maxi, and D-Tube96™

    Related Products & Applications

    Related Products

    Catalog Number Description
    71504 D-Tube™ Dialyzer Mini, MWCO 6-8 kDa
    71505 D-Tube™ Dialyzer Mini, MWCO 12-14 kDa
    71506 D-Tube™ Dialyzer Midi, MWCO 3.5 kDa
    71507 D-Tube™ Dialyzer Midi, MWCO 6-8 kDa
    71508 D-Tube™ Dialyzer Maxi, MWCO 3.5 kDa
    71509 D-Tube™ Dialyzer Maxi, MWCO 6-8 kDa

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    D-Tube™

    Categories

    Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Protein Dialyzers