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508741 DAPI Stain - CAS 28718-90-3 - Calbiochem

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl in H₂O.

DAPI Stain - CAS 28718-90-3 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O

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508741
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
28718-90-3C₁₆H₁₅N₅ • 2HCl

Products

Catalog NumberPackaging Qty/Pack
5.08741.0001 Glass bottle 5 mg
Description
OverviewCells are typically stained at 1 µg/ml.
Catalogue Number508741
Brand Family Calbiochem®
Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
References
ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.
Product Information
CAS number28718-90-3
FormLiquid
FormulationA 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
Hill FormulaC₁₆H₁₅N₅ • 2HCl
Chemical formulaC₁₆H₁₅N₅ • 2HCl
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ200
Applications
Biological Information
Primary TargetDNA
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.08741.0001 04055977261370

Documentation

DAPI Stain - CAS 28718-90-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-March-2014 JSW
Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
DescriptionCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
FormLiquid
FormulationA 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
Intert gas (Yes/No) Packaged under inert gas
CAS number28718-90-3
Chemical formulaC₁₆H₁₅N₅ • 2HCl
Structure formulaStructure formula
Purity≥97% by HPLC
Storage Protect from light
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
Hotz, M.A., et al. 1994. Cytometry 15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
Miller, T., et al. 1993. Biotechniques 15, 1042.
Gray, J.W., et al. 1982. Science 238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
Russels, W.C., et al. 1975. Nature 253, 461.