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509583 Diaphanous (mDia)-related Formin Agonist, IMM01 - CAS 218795-74-5

An agonist of mammalian Diaphanous (mDia)-related formins that disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC₅₀ = 140 nM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Formin Activator

Primary Target: mDia
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
218795-74-5C₁₂H₁₇N₃O₂S

Products

Catalog NumberPackaging Qty/Pack
5.09583.0001 Glass bottle 10 mg
Description
OverviewA hydrazinecarbothioamide derived intramimic that acts as an agonist of mammalian Diaphanous (mDia)-related formins and disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC50 = 140 nM). Shown to trigger actin assembly and stabilize microtubules in a dose -dependent manner and at levels similar to those induced by Taxol. Induces caspase-3 activation and apoptosis in NIH 3T3 and SW480 cells(~ 100 µM) and slows the growth of SW480 xenograft tumors in mice (~ 25 mg/kg). Shown to induce LacZ expression, producing b-gal activity that is comparable to effects seen with cytochalasin D treatment.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number509583
Brand Family Calbiochem®
SynonymsFormin Activator
References
ReferencesLash, L.L., et al. 2013. Cancer Res.73, 6793.
Copeland, S.J. et al. 2007. J Biol. Chem. 282, 30120.
Product Information
CAS number218795-74-5
FormOff-white powder
Hill FormulaC₁₂H₁₇N₃O₂S
Chemical formulaC₁₂H₁₇N₃O₂S
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetmDia
Primary Target IC<sub>50</sub>140 nM
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.09583.0001 04055977240962

Documentation

Diaphanous (mDia)-related Formin Agonist, IMM01 - CAS 218795-74-5 SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Lash, L.L., et al. 2013. Cancer Res.73, 6793.
Copeland, S.J. et al. 2007. J Biol. Chem. 282, 30120.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-March-2014 JSW
SynonymsFormin Activator
DescriptionA hydrazinecarbothioamide derived intramimic that acts as an agonist of mammalian Diaphanous (mDia)-related formins and disrupts diaphanous inhibitory domain (DID) and diaphanous autoregulatory domain (DAD) interaction (IC50 = 140 nM). Shown to trigger actin assembly and stabilize microtubules in a dose -dependent manner and at levels similar to those induced by Taxol. Induces caspase-3 activation and apoptosis in NIH 3T3 and SW480 cells(~ 100 µM) and slows the growth of SW480 xenograft tumors in mice (~ 25 mg/kg). Shown to induce LacZ expression, producing b-gal activity that is comparable to effects seen with cytochalasin D treatment.
FormOff-white powder
Intert gas (Yes/No) Packaged under inert gas
CAS number218795-74-5
Chemical formulaC₁₂H₁₇N₃O₂S
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesLash, L.L., et al. 2013. Cancer Res.73, 6793.
Copeland, S.J. et al. 2007. J Biol. Chem. 282, 30120.