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309800 Dihydroethidium - CAS 38483-26-0 - Calbiochem

A cell-permeable, chemically-reduced ethidium derivative.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE

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309800
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
38483-26-0C₂₁H₂₁N₃

Products

Catalog NumberPackaging Qty/Pack
309800-25MG Glass bottle 25 mg
Description
OverviewA cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
Catalogue Number309800
Brand Family Calbiochem®
Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
References
ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
Product Information
CAS number38483-26-0
ATP CompetitiveN
FormPink solid
Hill FormulaC₂₁H₂₁N₃
Chemical formulaC₂₁H₂₁N₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetUseful fluorogenic probe for the detection of reactive oxygen species (ROS)
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSSF7935000
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
309800-25MG 04055977216806

Documentation

Dihydroethidium - CAS 38483-26-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Dihydroethidium - CAS 38483-26-0 - Calbiochem Certificates of Analysis

TitleLot Number
309800

References

Reference overview
Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-June-2008 RFH
Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
DescriptionA cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.
FormPink solid
Intert gas (Yes/No) Packaged under inert gas
CAS number38483-26-0
RTECSSF7935000
Chemical formulaC₂₁H₂₁N₃
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMF or DMSO (20 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Irritant
ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.