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PMEF-DR4 EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain DR4, Mitomycin C Treated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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PMEF-DR4
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Key Specifications Table

Key Applications
Cult
Description
Catalogue NumberPMEF-DR4
Trade Name
  • Embryomax
  • Specialty Media
DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
OverviewPlating MEF Feeder Cells

Procedure:

1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Alternate Names
  • MEF Feeder Cells, DR4 PMEFs, DR4 MEFs, MEFs, Mouse Feeder Cells
Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

The DR4 strain of MEF feeder cells are resistant to neomycin, hygromycin, puromycin, and 6-thioguanine. They are mitotically arrested by mytomycin-C treatment and will not proliferate.
References
Product Information
Components
  • EmbryoMax PMEF, P3, Strain DR4, Mito-C Treated: (Part number PMEF-DR4) One (1) vial containing ≥4 x10^6 cells per vial.
Quality LevelMQ100
Applications
ApplicationPMEF, Strain DR4, Mitomycin C Treated, Passage 3
Key Applications
  • Stem Cell Culture
Biological Information
Cell Line Type
  • Primary Mouse Embryo Fibroblasts
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceCell Viability and Morphology: PASSED
Mycoplasma Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Storage and Shipping Information
Storage ConditionsVials should be stored in the vapor phase of liquid nitrogen.
Packaging Information
Material Size1 vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PMEF-DR4 04054839159992