Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

PMEF-N EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Neo Resistant, Strain FVB, Mitomycin C Treated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
View Products on Sigmaaldrich.com
PMEF-N
5 vials  5-6x106 ea
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

Interested in purchasing an entire lot of MEF’s?
Bulk sized lots of MEFs are available with lot sampling.
Order Today

Key Specifications Table

Key Applications
Cult
Description
Catalogue NumberPMEF-N
Trade Name
  • Embryomax
  • Specialty Media
DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
OverviewPrimary Mouse Embryo Fibroblasts, Neo resistant, strain FVB, are Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to neomycin.

Plating MEF Feeder Cells

Procedure:

1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Alternate Names
  • EmbryoMax® Primary Mouse Embryonic Fibroblasts
Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
References
Product Information
Quality LevelMQ100
Applications
ApplicationPMEF, Neo Resistant, Strain FVB, Mitomycin C Treated, Passage 3
Key Applications
  • Stem Cell Culture
Biological Information
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • PMEFs
  • Induced Pluripotent Stem Cells
Cell Line Type
  • Primary Mouse Embryo Fibroblasts
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Packaging Information
Material Size5 vials
Material Package5-6x106 ea
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PMEF-N 04053252722929

Documentation

Protocols

Title
General Experimental Outline for Targeting ES Cells
Plating PMEF Feeder Cells Protocol

EmbryoMax® Primary Mouse Embryonic Fibroblasts SDS

Title

Safety Data Sheet (SDS) 

EmbryoMax® Primary Mouse Embryonic Fibroblasts Certificates of Analysis

TitleLot Number
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 2023450 2023450
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 2028728-2 2028728-2
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 2037815-1 2037815-1
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 01113-2 01113-2
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 01120-2 01120-2
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 01120-4 01120-4
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 01211-3 01211-3
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 10108-3 10108-3
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 10108-4 10108-4
EmbryoMax#174; PMEF Cells Neo Resistant, Mito-C Treated - 10108-6 10108-6
Show more

Brochure

Title
Murine Embryonic Stem Cell Culture

Technical Info

Title
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling