Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

GF155 Epidermal Growth Factor Protein, Recombinant mouse

The Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal & epithelial cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P01133, P01132
View Products on Sigmaaldrich.com
GF155
500 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

SourcePurity
E.coliGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of EGF.
Description
Catalogue NumberGF155
ReplacesGF123
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionEpidermal Growth Factor Protein, Recombinant mouse
OverviewEpidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6.2 kDa protein containing 54 amino acid residues.
Alternate Names
  • EGF
References
Product Information
PresentationLyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.
Quality LevelMQ100
Applications
ApplicationThe Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal & epithelial cells.
Application NotesFor most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
Biological Information
PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of EGF.
SourceE.coli
Specific ActivityMouse EGF is fully biologically active when compared to standards. The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.15 ng/mL, corresponding to a specific activity of ≥ 6.6 x 10<sup>6</sup> units/mg.
Entrez Gene SummaryEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Gene Symbol
  • EGF
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01133 # EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
SIZE: 1207 amino acids; 133946 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P01133 ## Contains 9 EGF-like domains. & Contains 9 LDL-receptor class B repeats.
Stem Cell Type
  • Neural Stem Cells
  • Epithelial Cells
  • Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
GF155 04053252676178

Documentation

Epidermal Growth Factor Protein, Recombinant mouse Certificates of Analysis

TitleLot Number
EGF, MOUSE RECOMBINANT PROTEIN - 2382128 2382128
EGF, MOUSE RECOMBINANT PROTEIN - 2391023 2391023
EGF, MOUSE RECOMBINANT PROTEIN - 2074901 2074901
EGF, MOUSE RECOMBINANT PROTEIN - 2958519 2958519
EGF, MOUSE RECOMBINANT PROTEIN - 3760637 3760637
EGF, MOUSE RECOMBINANT PROTEIN - DAM1766162 DAM1766162
EGF, MOUSE RECOMBINANT PROTEIN - JBC1865898 JBC1865898
EGF, MOUSE RECOMBINANT PROTEIN -2574605 2574605
EGF, MOUSE RECOMBINANT PROTEIN -2658911 2658911
EGF, MOUSE RECOMBINANT PROTEIN -2682528 2682528
Show more

Data Sheet

Title
EGF, MOUSE RECOMBINANT PROTEIN

Newsletters / Publications

Title
Cellutions Newsletter: 2007, Volume 1
Cellutions Newsletter: 2008, Volume 1
Cellutions Newsletter: 2008, Volume 3

Related Products & Applications

Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors