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GF321 FGF-acidic, Human Recombinant
Animal Free

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P05230
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GF321
50µg  
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Overview

Replacement Information

Key Specifications Table

SourcePurity
Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.		Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Description
Catalogue NumberGF321
DescriptionFGF-acidic, Human Recombinant
Animal Free
Alternate Names
  • FGF-1
  • alpha FGF
  • FGFa
Background InformationFGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGFacidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGFacidic is a 16.0 kDa protein consisting of 141 amino acid residues.
References
Product Information
PresentationProduct is presented in 5 mM Sodium Phosphate, pH 8.0 + 100mM NaCl. and is filtered through a 0.2 micron filter before lyophilization.
Quality LevelMQ100
Applications
Biological Information
PurityProduct endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
SourceProtein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceBioactivity assay:
Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml corresponding to a specific activity of ≥ 1 x 10^5 units/mg.
Assay #2: The ED50 was determined by a cell proliferation
assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence
of 10 μg/ml heparin, corresponding to a specific activity of
≥ 2 x 10^6 units/mg.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in 5 mM Sodium Phosphate, pH 8.0 to a concentration of < 0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Packaging Information
Material Size50µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
GF321 04053252979613

Documentation

FGF-acidic, Human Recombinant
Animal Free SDS

Title

Safety Data Sheet (SDS) 

FGF-acidic, Human Recombinant
Animal Free Certificates of Analysis

TitleLot Number
FGF-acidic, Human Recombinant Animal Free - Q2441380 Q2441380
FGF-acidic, Human Recombinant Animal Free 2475923
FGF-acidic, Human Recombinant Animal Free - 3237545 3237545
FGF-acidic, Human Recombinant Animal Free - 3381375 3381375
FGF-acidic, Human Recombinant Animal Free - 3439357 3439357
FGF-acidic, Human Recombinant Animal Free - 3533573 3533573
FGF-acidic, Human Recombinant Animal Free - 3739703 3739703
FGF-acidic, Human Recombinant Animal Free - 4004972 4004972

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