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PF092 Fas Ligand Plus, Human, Recombinant

Fas Ligand Plus, Human, Recombinant, consists of amino acids 103-281 fused to a 26-amino acid linker and FLAG®-tag at the N-terminus and expressed in HEK293 cells.

Fas Ligand Plus, Human, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PF092
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
PF092-5UG Plastic ampoule 5 μg
Description
OverviewRecombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG®-tag at the N-terminus and expressed in HEK293 cells. Does not require enhancer for activity. Suitable for cytotoxicity assays. M.W. 32,000 - 35,000.
Catalogue NumberPF092
Brand Family Calbiochem®
References
ReferencesAshkenazi, A., and Dixit, V.M. 1998. Science 281, 1305.
Nagata, S., 1997 Cell 88, 355.
Hahne, M., et al. 1996. Science 274, 1363.
Bellgrau, D., et al. 1995. Nature 377, 630.
Lowin, B., et al. 1994. Nature 370, 650.
Russell, J.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4409.
Product Information
FormLyophilized
FormulationLyophilized from PBS.
Quality LevelMQ100
Applications
Application CommentsGlycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~35 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity.
Biological Information
Biological activity≥1 ng/ml kills Fas-sensitive cells
Purity≥95% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PF092-5UG 04055977207491

Documentation

Fas Ligand Plus, Human, Recombinant SDS

Title

Safety Data Sheet (SDS) 

Fas Ligand Plus, Human, Recombinant Certificates of Analysis

TitleLot Number
PF092

References

Reference overview
Ashkenazi, A., and Dixit, V.M. 1998. Science 281, 1305.
Nagata, S., 1997 Cell 88, 355.
Hahne, M., et al. 1996. Science 274, 1363.
Bellgrau, D., et al. 1995. Nature 377, 630.
Lowin, B., et al. 1994. Nature 370, 650.
Russell, J.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4409.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-July-2008 JSW
DescriptionRecombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG®-tag at the N-terminus and expressed in HEK293 cells. Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~37-42 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity. Useful for cytotoxicity assays.

Fas Ligand (FasL/APO-1L/CD95L) is a ~37-42 kDa type II transmembrane protein belonging to the TNF family. Interaction between FasL and Fas induces apoptosis of Fas-sensitive cells. FasL is implicated in CTL-mediated-killing, activation-induced cell death, creation of immune-privileged sites and tissue homeostasis. The extracellular domain of human FasL can be cleaved off by a metalloprotease, generating soluble FasL (sFasL). Circulating sFasL can be detected in serum of patients with melanoma and certain types of lymphoma.
BackgroundFas Ligand (FasL/APO-1L/CD95L) is a ~40 kDa type II transmembrane protein belonging to the TNF family. Interaction between FasL and Fas induces apoptosis of Fas-sensitive cells. FasL is implicated in CTL-mediated-killing, activation-induced cell death, creation of immune-privileged sites and tissue homeostasis. The extracellular domain of human FasL can be cleaved off by a metalloprotease, generating soluble FasL (sFasL). Circulating sFasL can be detected in serum of patients with melanoma and certain types of lymphoma.
FormLyophilized
FormulationLyophilized from PBS.
Purity≥95% by SDS-PAGE
Biological activity≥1 ng/ml kills Fas-sensitive cells
SolubilityReconstitute in 50 µl PBS to yield a final stock concentration of 100 µg/ml. Further dilute in culture medium with 5% FCS immediately prior to use.
CommentsGlycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~35 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesAshkenazi, A., and Dixit, V.M. 1998. Science 281, 1305.
Nagata, S., 1997 Cell 88, 355.
Hahne, M., et al. 1996. Science 274, 1363.
Bellgrau, D., et al. 1995. Nature 377, 630.
Lowin, B., et al. 1994. Nature 370, 650.
Russell, J.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4409.