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70850 FastPlax™ Titer Kit - Novagen

Rapid, reliable titering of baculovirus stocks

FastPlax™ Titer Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70850
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
70850-3 Plastic ampoule 5 ea
Description
OverviewThe FastPlax™ Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.

The FastPlax Antibody is used to detect the appearance of AcNPV gp64 glycoprotein on the surface of infectted cells as early as 8-24 hours post-infection. This forms the basis of the FastPlax Titer Kit, which allows determination of baculovirus titers in just 25-48 hours.

The FastPlax Antibody can also be used to detect gp64 on Western blots.
Catalogue Number70850
Brand Family Novagen®
References
Product Information
•2 × 50 ml10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween® 20)
•6 ml10% Gelatin (contains 0.02% azide)
•10 µlFastPlax Antibody
•300 µlGoat-Anti-Mouse β-Galactosidase Conjugate
•300 µlNBT (83 mg/ml in 70% dimethylformamide)
•300 µlX-gal (50 mg/ml in dimethylformamide)
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
70850-3 04055977272642