Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

SCT154 Firefly Luciferase Assay

Flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.

View Products on Sigmaaldrich.com
SCT154
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT154
DescriptionFirefly Luciferase Assay
OverviewFirefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.

The Firefly Luciferase Assay is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. The luminescence signal decays over the course of about 10 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels.
Alternate Names
  • Firefly Luciferase Assay
  • Bioluminescent Assay
References
Product Information
Components
  • 1) 5X Firefly Luciferase Lysis Buffer (CS224525): 2 X 15 mL
  • 2) Firefly Luciferase Assay Buffer 2.0 (CS224585): 100 mL
  • 3) D-Luciferin (CS224519): 2 X 10 mg
Detection methodChemiluminescence
Presentationliquid
Applications
ApplicationFlash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.
Key Applications
  • Cell Based Assays
Application NotesFirefly Luciferase Assay
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore Firefly Luciferase Assay at -80°C. Firefly Luciferase Assay Buffer 2.0 is stable at -20°C for three months and at –80°C for at least six months from date of receipt. The other kit components are stable at -20°C for at least six months from date of receipt. Kit components and D-luciferin stock solutions in water are stable to at least 5 freeze-thaw cycles.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
SCT154 04054839566066