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344291 Freund’s Incomplete Adjuvant, Modified

Freund’s Incomplete Adjuvant, Modified Contains 85% Drakeol 5NF, 15% Arlacel A. Heat sterilized. Contains zero colony forming units.

Freund’s Incomplete Adjuvant, Modified MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
344291-10ML Glass bottle 10 ml
Description
OverviewContains 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier). Heat sterilized. Contains zero colony forming units/ml.
Catalogue Number344291
Brand Family Calbiochem®
References
ReferencesFreund, J. 1947. Ann. Rev. Microbiol. 1, 291.
Product Information
FormYellow oily liquid
Quality LevelMQ100
Applications
ApplicationFreund’s Incomplete Adjuvant, Modified Contains 85% Drakeol 5NF, 15% Arlacel A. Heat sterilized. Contains zero colony forming units.
Application Comments10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

85% Drakeol 5 NF (light mineral oil)
15% Arlacel A, mannide monooleate emulsifier.

1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
344291-10ML 04055977214703

Documentation

Freund’s Incomplete Adjuvant, Modified SDS

Title

Safety Data Sheet (SDS) 

Freund’s Incomplete Adjuvant, Modified Certificates of Analysis

TitleLot Number
344291

References

Reference overview
Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-June-2008 RFH
DescriptionThe word "adjuvant" is derived from the Latin, adjuvare, meaning "to help." During immunization, an adjuvant is generally mixed with the antigen to enhance the rate and amount of antibody formed. The most popular complete adjuvant is that of Freund. It consists of killed and dried microbial cells suspended in an emulsifying oil. Adjuvant without the microbial cells is called "incomplete adjuvant." Complete adjuvant forms a stable water-in-oil emulsion with aqueous solution of antigen. When injected subcutaneously into animals, the emulsified antigen forms a depot from which antigen is delivered slowly and at sustained levels to the animal's antibody-synthesizing system. The induced antibody response is usually high and sustained. In guinea pigs, an additional benefit from adjuvant is the delayed appearance of hypersensitivity.
FormYellow oily liquid
SolubilityComposition
10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:
85% Drakeol 5 NF (light mineral oil)
15% Arlacel A, mannide monooleate emulsifier

Sample Preparation
1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Comments10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

85% Drakeol 5 NF (light mineral oil)
15% Arlacel A, mannide monooleate emulsifier.

1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesFreund, J. 1947. Ann. Rev. Microbiol. 1, 291.