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345834 Genistein, Soybean - CAS 446-72-0 - Calbiochem

A cell-permeable, reversible, substrate competitive inhibitor of protein tyrosine kinases, including autophosphorylation of epidermal growth factor receptor kinase (IC₅₀ = 2.6 µM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 4ʹ,5,7-Trihydroxyisoflavone

Primary Target: EGFR kinase
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
446-72-0C₁₅H₁₀O₅

Products

Catalog NumberPackaging Qty/Pack
345834-20MG Glass bottle 20 mg
345834-50MG Plastic ampoule 50 mg
Description
OverviewA cell-permeable, reversible, substrate competitive inhibitor of protein tyrosine kinases, including autophosphorylation of epidermal growth factor receptor kinase (IC50 = 2.6 µM). The inhibition is competitive with respect to ATP and non-competitive with respect to the phosphate acceptor. Has only a trivial effect on the activity of PKA and PKC (IC50 > 350 µM). Inhibits tumor cell proliferation and induces tumor cell differentiation. Produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells. However, it prevents anti-CD3 monoclonal antibody-induced thymic apoptosis. Also inhibits topoisomerase II activity in vitro.
Catalogue Number345834
Brand Family Calbiochem®
Synonyms4ʹ,5,7-Trihydroxyisoflavone
References
ReferencesConstantinou, A., and Huberman, E. 1995. Proc. Soc. Exp. Biol. Med. 208, 109.
Wei, H., et al. 1995. Proc. Soc. Exp. Biol. Med. 208, 124.
Wei, H., et al. 1995. Carcinogenesis 17, 73.
Kobayashi, S., et al. 1994. J. Biol. Chem. 269, 9011.
Migita, K., et al. 1994. J. Immunol. 153, 3457.
Spinozzi, F., et al. 1994. Leuk. Res. 18, 431.
Dhar, A., et al. 1990. Mol. Pharmacol. 37, 519.
Hill, T.D., et al. 1990. Science 248, 1660.
Dean, N.M., et al. 1989. Biochem. Biophys. Res. Commun. 165, 795.
Akiyama, T., et al. 1987. J. Biol. Chem. 262, 5592.
Product Information
CAS number446-72-0
ATP CompetitiveN
FormWhite solid
Hill FormulaC₁₅H₁₀O₅
Chemical formulaC₁₅H₁₀O₅
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetEGFR kinase
Primary Target IC<sub>50</sub>2.6 µM against autophosphorylation of epidermal growth factor receptor kinase
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNR2392000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
345834-20MG 04055977214376
345834-50MG 04055977214383

Documentation

Genistein, Soybean - CAS 446-72-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Genistein, Soybean - CAS 446-72-0 - Calbiochem Certificates of Analysis

TitleLot Number
345834

References

Reference overview
Constantinou, A., and Huberman, E. 1995. Proc. Soc. Exp. Biol. Med. 208, 109.
Wei, H., et al. 1995. Proc. Soc. Exp. Biol. Med. 208, 124.
Wei, H., et al. 1995. Carcinogenesis 17, 73.
Kobayashi, S., et al. 1994. J. Biol. Chem. 269, 9011.
Migita, K., et al. 1994. J. Immunol. 153, 3457.
Spinozzi, F., et al. 1994. Leuk. Res. 18, 431.
Dhar, A., et al. 1990. Mol. Pharmacol. 37, 519.
Hill, T.D., et al. 1990. Science 248, 1660.
Dean, N.M., et al. 1989. Biochem. Biophys. Res. Commun. 165, 795.
Akiyama, T., et al. 1987. J. Biol. Chem. 262, 5592.

Citations

Title
  • Weijian Shao, Roy C. Orlando and Mouhamed S. Awayda. (2005) Bisphosphonates stimulate an endogenous nonselective cation channel in Xenopus oocytes: potential mechanism of action. American Journal of Physiology Cell Physiology 289, C248-C256.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision16-April-2010 JSW
    Synonyms4ʹ,5,7-Trihydroxyisoflavone
    DescriptionA cell-permeable, reversible, substrate competitive inhibitor of protein tyrosine kinases, including autophosphorylation of epidermal growth factor receptor kinase (IC50 = 2.6 µM). The inhibition is competitive with respect to ATP and non-competitive with respect to the phosphate acceptor. Inhibits tumor cell proliferation and induces tumor cell differentiation. Inhibits exogenous substrate phosphorylation by EGFR kinase and pp60v-src with similar potencies (IC50 = 20-25 µM). Has only a trivial effect on PKC and PKA (IC50 >350 µM). Produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells. Prevents anti-CD3 monoclonal antibody-induced thymic apoptosis. Also inhibits topoisomerase II activity in vitro. Blocks oxidative DNA damage in vitro.
    FormWhite solid
    CAS number446-72-0
    RTECSNR2392000
    Chemical formulaC₁₅H₁₀O₅
    Structure formulaStructure formula
    Purity≥95% by HPLC
    SolubilityDMSO (200 mg/ml)
    Storage Protect from light
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
    Toxicity Standard Handling
    Merck USA index14, 4391
    ReferencesConstantinou, A., and Huberman, E. 1995. Proc. Soc. Exp. Biol. Med. 208, 109.
    Wei, H., et al. 1995. Proc. Soc. Exp. Biol. Med. 208, 124.
    Wei, H., et al. 1995. Carcinogenesis 17, 73.
    Kobayashi, S., et al. 1994. J. Biol. Chem. 269, 9011.
    Migita, K., et al. 1994. J. Immunol. 153, 3457.
    Spinozzi, F., et al. 1994. Leuk. Res. 18, 431.
    Dhar, A., et al. 1990. Mol. Pharmacol. 37, 519.
    Hill, T.D., et al. 1990. Science 248, 1660.
    Dean, N.M., et al. 1989. Biochem. Biophys. Res. Commun. 165, 795.
    Akiyama, T., et al. 1987. J. Biol. Chem. 262, 5592.
    Citation
  • Weijian Shao, Roy C. Orlando and Mouhamed S. Awayda. (2005) Bisphosphonates stimulate an endogenous nonselective cation channel in Xenopus oocytes: potential mechanism of action. American Journal of Physiology Cell Physiology 289, C248-C256.
    		•