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109217 Gram's safranine solution

for the Gram staining method

Gram's safranine solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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109217
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
1092170500 Plastic bottle 500 ml
1092172500 Plastic bottle 2.5 l
Description
Catalogue Number109217
DescriptionGram's safranine solution
OverviewThis ready-to-use Gram's safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear pink to red.
A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no.1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor the Gram staining method
Biological Information
Physicochemical Information
Density0.98 g/cm3 (20 °C)
Flash point49 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H226: Flammable liquid and vapour.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P240: Ground and bond container and receiving equipment.
P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
P242: Use non-sparking tools.
P243: Take action to prevent static discharges.
Signal WordWarning
Storage class3 Flammable liquids
WGKWGK 1 slightly hazardous to water
Disposal28
Aqueous solutions: Container D.
Safety Information
Categories of dangerflammable
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDKein Gefahrgut
Declaration (transport by air) IATA-DGRNo Dangerous Good
Declaration (transport by sea) IMDG-CodeNo Dangerous Good
Supplemental Information
Specifications
Suitability for microscopypasses test
gram-positve microorganismsblue-violet
gram-negative microorganismspink to red
Global Trade Item Number
Catalog Number GTIN
1092170500 04022536112046
1092172500 04022536557397

Related Products & Applications

Accessories (Primary)

Catalog Number Description
100567 Lugol's solution
109261 Lugol's solution (diluted iodine-potassium iodide solution)
110218 Gram's decolorizing solution

Accessories (Secondary)

Catalog Number Description
100567 Lugol's solution
109261 Lugol's solution (diluted iodine-potassium iodide solution)

Related Products By: Application Facete

Counter Staining
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Bacteria Staining

Categories

IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining