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SCT065 Green-AlDeSense™ ALDH1A1 Live Cell Dye

ALDH1A1 isoform specific live cell dye which labels cancer stem cells for fluorescent microscopy, flow cytometry and in vivo applications.

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SCT065
1/Kit  
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Overview

Replacement Information
Description
Catalogue NumberSCT065
DescriptionGreen-AlDeSense™ ALDH1A1 Live Cell Dye
OverviewCancer stem cells (CSCs) have an increased capacity to activate antiapoptotic/prosurvival pathways as well as overexpressing ATP-binding cassette transporters which act as potent efflux pumps for potential chemotherapeutics. Aldehyde dehydrogenases (ALDH), in particular the ALDH1A1 isoform, is believed to be a reliable marker of CSCs across many cancer types, including prostate, lung, breast, esophageal, and ovarian cancers. ALDH1A1 is associated with increased chemotherapy resistance and poor clinical outcome and is an established stem cell marker.

Current methods to identify ALDH in living cells are limited by cross-reactivity with several ALDH isoforms producing difficulties in data analysis and prognosis. Chan, et al. have created a ALDH1A1 isoform specific live cell dye termed Green-AlDeSense™ which labels cancer stem cells and exhibits a 20-fold fluorescent enhancement when bound to ALDH1A1. The dye identifies ALDH1A1 positive CSCs using live cell imaging, flow cytometry and in vivo studies.

Spectral Properties: absmax: 496 nm, emmax: 516 nm.

Reference

1) Chan, et al. Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1. ACS Cent Sci. 2018 Aug 22;4(8):1045-1055.
References
Product Information
Components
  • The Green-AlDeSense™ ALDH1A1 Live Cell Dye Kit (SCT065) contains:
  • 1) Ctrl-Green-AlDeSense™ Dye (CS226256), 100 µg. Store at -20°C. Protect from light. MW: 380.3 g/mol.
  • 2) Green-AlDeSense™ Dye (CS226257), 100 µg. Store at -20°C. Protect from light. MW: 438.4 g/mol.
Detection methodFluorescence
HS Code3204 90 00
Applications
ApplicationALDH1A1 isoform specific live cell dye which labels cancer stem cells for fluorescent microscopy, flow cytometry and in vivo applications.
Key Applications
  • Flow Cytometry
  • Fluorescence
  • Cell Based Assays
Biological Information
Gene Symbol
  • ALDH1A1
Molecular WeightCtrl-Green-AlDeSense™ Dye: 380.3 g/mol.
Green-AlDeSense™ Dye: 438.4 g/mol.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceColor: Orange
Form: Solid
Solubility: DMSO: >10 mg/mL
Purity: ≥ 80.0%
Elemental Analysis/Identification: Conforms
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20ﾟC. Protect from light.
Packaging Information
Material Size1/Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
SCT065 04061841849773