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03-227 HDAC3/NCOR1 complex human recombinant

Human recombinant HDAC3/NCOR1 produced in insect cells.

HDAC3/NCOR1 complex human recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
03-227
50 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Key ApplicationsSpecies
Enzyme AssaysHuman
Description
Catalogue Number03-227
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHDAC3/NCOR1 complex human recombinant
OverviewHuman recombinant HDAC3/NCOR1 produced in insect cells
Background InformationUnlike other class I HDAC enzymes, HDAC3 is found in co-repressor complexes with the SMRT/N-CoR proteins. HDAC3 contains a unique domain structure which includes both nuclear localization and nuclear export sequences. HDAC3 has been implicated in cancer, as HDAC3 inhibitors produce anti-proliferative effects in various cancer cells. It may also play a role in heart disease, which may involve deacetylation of MEF2.
References
Product Information
FormatPurified
PresentationIn liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant HDAC3/NCOR1 produced in insect cells.
Key Applications
  • Enzyme Assays
Application NotesHDAC deacetylase assays
Biological Information
PurityPurity >90% by SDS-PAGE
SourceHuman HDAC3 and the activation domain of human NCOR1
Specific Activity>150 U/µg (based on total protein;>250 U/µg based on weight of HDAC3). 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
Molecular Weight49/40 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC3/NCOR1 into separate tubes before storing at -80°C.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-227 04053252550881

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