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391338 HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.05.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid

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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
7365-45-9C₈H₁₈N₂O₄S

Products

Catalog NumberPackaging Qty/Pack
391338-100GM Glass bottle 100 gm
391338-1KG Glass bottle 1 kg
391338-25GM Plastic ampoule 25 gm
391338-500GM Glass bottle 500 gm
391338-5KG Fibre carton 5 kg
Description
OverviewA zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
Absorbance (1.0 M, H2O, 260 nm):≤0.05.
Absorbance (1.0 M, H2O, 280 nm): ≤0.04
Catalogue Number391338
Brand Family Calbiochem®
SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
References
Product Information
CAS number7365-45-9
FormWhite crystalline powder
Hill FormulaC₈H₁₈N₂O₄S
Chemical formulaC₈H₁₈N₂O₄S
PKA7.25 - 7.75 at 20°C
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥99% by titration (dry basis)
Physicochemical Information
ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
Heavy metals≤1 ppm
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSTL6809000
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-37/39

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable gloves and eye/face protection.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
391338-100GM 07790788049805
391338-1KG 07790788049812
391338-25GM 04055977190168
391338-500GM 07790788049829
391338-5KG 04055977212419

Documentation

HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem Certificates of Analysis

TitleLot Number
391338

Brochure

Title
Bulk packaging

Technical Info

Title
Molecular Biology Grade Reagents
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-October-2009 RFH
SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
DescriptionA zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
FormWhite crystalline powder
CAS number7365-45-9
RTECSTL6809000
Chemical formulaC₈H₁₈N₂O₄S
Structure formulaStructure formula
Purity≥99% by titration (dry basis)
ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
SolubilityH₂O (1 M)
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
Toxicity Irritant
Merck USA index14, 4654