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533761 HSF1 Activator HSF1A - CAS 1196723-93-9 - Calbiochem

HSF1 Activator, HSF1A, CAS 1196723-93-9, is a cell-permeable activator of HSF1 that protects mammalian cells against stress-induced apoptosis. Protects cells from polyQ cytotoxicity.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Heat Shock Factor 1 Activator, HSF1A

Primary Target: HSF1
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
1196723-93-9C₂₁H₁₉N₃O₂S₂

Products

Catalog NumberPackaging Qty/Pack
5.33761.0001 Glass bottle 25 mg
Description
OverviewA cell-permeable pyrazolylsulfonamide compound that activates heat shock factor 1 (HSF1) and protects mammalian cells against stress-induced apoptosis. Offers protection against ER stress-induced apoptosis as indicated by blunting of caspase-3 activity in treated cells. Shown to activate human HSF1 function in yeast cells. Promotes the nuclear localization and phosphorylation of HSF1 (within 2 h of treatment). Activates Hsp25 and Hsp70 expression in wild-type mouse embryonic fibroblasts (~ 30 µM) and Hsp70 in HeLa cells. Shown to directly and reversibly interact with cytosolic chaperonin TRiC/CCT subunit (Kd = 613 nM for HSF1A-FITC and Tcp1), involving the hinge 2 region, and destabilize TRiC/CCT-HSF1 association. However, it does not perturb ATP hydrolysis. Reduces protein misfolding and cell death in PolyQ-expressing neuronal precursor cells and protects against cytotoxicity in Drosophila melanogaster model of PolyQ-mediated neurodegeneration.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
Catalogue Number533761
Brand Family Calbiochem®
SynonymsHeat Shock Factor 1 Activator, HSF1A
References
ReferencesNeef, D.W., et al. 2014. Cell Reports. 9, 955.
Neef, D.W., et al. 2010. PLoS Biology. 8, e1000291.
Product Information
CAS number1196723-93-9
FormOff-white solid
Hill FormulaC₂₁H₁₉N₃O₂S₂
Chemical formulaC₂₁H₁₉N₃O₂S₂
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetHSF1
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.33761.0001 04055977286700

Documentation

HSF1 Activator HSF1A - CAS 1196723-93-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

HSF1 Activator HSF1A - CAS 1196723-93-9 - Calbiochem Certificates of Analysis

TitleLot Number
533761

References

Reference overview
Neef, D.W., et al. 2014. Cell Reports. 9, 955.
Neef, D.W., et al. 2010. PLoS Biology. 8, e1000291.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-November-2015 JSW
SynonymsHeat Shock Factor 1 Activator, HSF1A
DescriptionA cell-permeable pyrazolylsulfonamide compound that activates heat shock factor 1 (HSF1) and protects mammalian cells against stress-induced apoptosis. Offers protection against ER stress-induced apoptosis as indicated by blunting of caspase-3 activity in treated cells. Shown to activate human HSF1 function in yeast cells. Promotes the nuclear localization and phosphorylation of HSF1 (within 2 h of treatment). Activates Hsp25 and Hsp70 expression in wild-type mouse embryonic fibroblasts (~ 30 µM) and Hsp70 in HeLa cells. Shown to directly and reversibly interact with cytosolic chaperonin TRiC/CCT subunit (Kd = 613 nM for HSF1A-FITC and Tcp1), involving the hinge 2 region, and destabilize TRiC/CCT-HSF1 association. However, it does not perturb ATP hydrolysis. Reduces protein misfolding and cell death in PolyQ-expressing neuronal precursor cells and protects against cytotoxicity in Drosophila melanogaster model of PolyQ-mediated neurodegeneration.
FormOff-white solid
Intert gas (Yes/No) Packaged under inert gas
CAS number1196723-93-9
Chemical formulaC₂₁H₁₉N₃O₂S₂
Purity≥98% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesNeef, D.W., et al. 2014. Cell Reports. 9, 955.
Neef, D.W., et al. 2010. PLoS Biology. 8, e1000291.