Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

EZBRAIN-SET Human Amyloid β40 and Amyloid β42 Brain ELISA

This Human Amyloid β40 & Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β40 & Amyloid β42 levels in Neuroscience research.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Colorimetric Research Category: Neuroscience Research Sub-Category: Alzheimer's Disease
View Products on Sigmaaldrich.com
EZBRAIN-SET
96-well plate  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Amyloid β40 Amyloid β42 HELISAColorimetric
Description
Catalogue NumberEZBRAIN-SET
DescriptionHuman Amyloid β40 and Amyloid β42 Brain ELISA
Background InformationAmyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β40 and Amyloid β42 Brain ELISA kits are used for the measurement of Amyloid beta 40 and Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. These assays are for research use only and appropriate for the in vitro detection of human Amyloid β40 and Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts
References
Product Information
Detection methodColorimetric
Precision, %
  • Intra-assay <10
  • Inter-assay <10
Quality LevelMQ200
Applications
ApplicationThis Human Amyloid β40 & Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β40 & Amyloid β42 levels in Neuroscience research.
Key Applications
  • ELISA
Application NotesThese assays require 50 µl of sample and are overnight assays.
Biological Information
SpecificityThe Amyloid β40 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

The Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.
Species Reactivity
  • Human
Analytes Available
  • Amyloid β40
  • Amyloid β42
Gene Symbol
  • APP
Physicochemical Information
Sensitivity
  • The lowest level of Amyloid β1-40 standard used in this assay is 6.0 pg/mL (50 μL sample size).
  • The lowest level of Amyloid ß1-42 standard used in this assay is 8 pg/mL (50 μL sample size).
Linearity of DilutionContact technical support for information
Standard Curve Range
  • 16 pg/mL – 500 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Storage ConditionsComponents in the kit can be stored up to 2 weeks at 2-8°C
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
EZBRAIN-SET 04053252005558