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EZBRAIN42 Human Amyloid β42 Brain ELISA

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EZBRAIN42
96-well plate  
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Amyloid β42 HELISAColorimetric
Description
Catalogue NumberEZBRAIN42
DescriptionHuman Amyloid β42 Brain ELISA
Background InformationAmyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.
References
Product Information
Detection methodColorimetric
Precision, %
  • Intra-assay <10
  • Inter-assay <10
Quality LevelMQ200
Applications
ApplicationThis Human Amyloid β42 Brain ELISA is used to measure & quantify Amyloid β42 levels in Neuroscience research.
Key Applications
  • ELISA
Application NotesThis assay requires 50 µl of sample and is an overnight assay.
Biological Information
SpecificityThe Amyloid β42 ELISA uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.
Species Reactivity
  • Human
Analytes Available
  • Amyloid β42
Gene Symbol
  • APP
Physicochemical Information
Sensitivity
  • The lowest level of Amyloid ß1-42 standard used in this assay is 8 pg/mL (50 μL sample size).
Linearity of DilutionContact technical support for information
Standard Curve Range
  • 16 pg/mL – 500 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Storage ConditionsComponents in the kit can be stored up to 2 weeks at 2-8°C
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
EZBRAIN42 04053252469688

Documentation

Protocols

Title
High Sensitivity Human Amyloid β42 ELISA
Protocol- EZBRAIN42

Human Amyloid β42 Brain ELISA SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms: Beyond fit for purpose
Custom Immunoassay Menu of Services

Technical Info

Title
96 well Template 2 Up
96 well Template Single
QUALITY CONTROL RANGES: High Sensitivity Human Amyloid Catalog# EZHS42
Quality Control Range Sheet- 2QC-112 and 2QC-212

Data Sheet

Title
Full Service Custom-built Assays