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EZGRA-88K Human Ghrelin (active) ELISA

This Human Ghrelin (active) ELISA is used to measure & quantify Ghrelin (active) levels in Metabolism & Endocrine & Neuroscience research.

Human Ghrelin (active) ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
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EZGRA-88K
96-well strip plate  
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Ghrelin (Active) HELISAFluorescent Colorimetric
Description
Catalogue NumberEZGRA-88K
DescriptionHuman Ghrelin (active) ELISA
Background InformationCirculating ghrelin is a multifunctional hormone secreted primarily by the stomach.
References
Product Information
Detection methodFluorescent Colorimetric
HS Code3002 15 90
Precision, %
  • Inter-assay: Serum: 3.5–6.6% Plasma: 7.5–12.9%
  • Intra-assay: Serum: 1.6–3.6% Plasma: 0.9–7.5%
Quality LevelMQ200
Applications
ApplicationThis Human Ghrelin (active) ELISA is used to measure & quantify Ghrelin (active) levels in Metabolism & Endocrine & Neuroscience research.
Key Applications
  • ELISA
Application NotesRoom temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Biological Information
SpecificityHuman Ghrelin (active) 100% Des-Octanoyl Human Ghrelin not detectable Canine Ghrelin (active) 106% Porcine Ghrelin (active) 57% Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
Species Reactivity
  • Human
Analytes Available
  • Ghrelin (Active)
Entrez Gene Number
Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
Gene Symbol
  • GHRL
  • ghrelin
  • obestatin
  • MTLRP
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
SIZE: 117 amino acids; 12911 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
Accuracy
  • Serum: 84.4–88.4%
  • Plasma: 94.7–94.8%
Physicochemical Information
Sensitivity
  • 8 pg/mL (20 µL sample size)
Linearity of DilutionSerum: 100.1–110.2% Plasma: 87.5–89.9%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is not available for sale in Japan.
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
EZGRA-88K 08436037123016

Documentation

Protocols

Title
Protocol- EZGRA-88K|EZGRA-88BK
Protocol: Human Ghrelin (Active)

Human Ghrelin (active) ELISA SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms: Beyond fit for purpose
Custom Immunoassay Menu of Services

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Range Sheet- AGR-117 and 217
Quality Control Range Sheet- AGR-118 and 218
Quality Control Ranges: Human Ghrelin (Active) ELISA - 96-Well Plate

Data Sheet

Title
Full Service Custom-built Assays

Related Products & Applications

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Catalog Number Description
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Categories

Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits