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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

EZHL-80BK Sigma-AldrichHuman Leptin "Dual Range" ELISA- Space Saver (Bulk) Packaging

This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders

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Overview

Replacement Information

Key Specifications Table

Analytes Available	Species Reactivity	Key Applications	Detection Methods
Leptin	H	ELISA	Fluorescent Colorimetric

Description
Catalogue Number	EZHL-80BK
Description	Human Leptin "Dual Range" ELISA- Space Saver (Bulk) Packaging
Background Information	Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.

References

Product Information
Detection method	Fluorescent Colorimetric
Precision, %	Inter-assay: 2.6–6.2 Sensitive Assay 1.3–8.6 Intra-assay: 2.6–4.6 Sensitive Assay 1.4–4.9
Quality Level	MQ200

Applications
Application	This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.
Key Applications	ELISA
Application Notes	Room temperature, 3.5 hour assay 25 μL sample size (Sensitive Assay: 50μL)

Biological Information
Species Reactivity	Human
Analytes Available	Leptin
Entrez Gene Number	NM_000230.1
Entrez Gene Summary	This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
Gene Symbol	LEP leptin OB obesity OBS
UniProt Number	P41159
UniProt Summary	FUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. SIZE: 167 amino acids; 18641 Da SUBUNIT: Interacts with SIGLEC6. SUBCELLULAR LOCATION: Secreted. DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665]. SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
Accuracy	94–114% Sensitive Assay 86–102%

Physicochemical Information
Sensitivity	0.5 ng/mL Sensitive Assay 0.125 ng/mL
Linearity of Dilution	80–105% Sensitive Assay 96–125%
Standard Curve Range	0.5–100 ng/mL Sensitive Assay 0.125–20 ng/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	For research use only. Not for use in diagnostic procedures.

Storage and Shipping Information

Packaging Information
Material Size	96-well strip plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
EZHL-80BK	04053252436963

Documentation

Human Leptin "Dual Range" ELISA- Space Saver (Bulk) Packaging SDS

Title
Safety Data Sheet (SDS)

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