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SCR502 Human, iPS Selection Kit

The Human iPS Selection Kit allows live cell imaging & identification of fully reprogrammed human induced pluripotent Stem cells from a heterogeneous population of reprogramming intermediates.

Human, iPS Selection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Immunocytochemistry
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SCR502
1 kit  Sufficient for 40 tests per antibody
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Overview

Replacement Information

Key Specifications Table

Key Applications
ICC
Description
Catalogue NumberSCR502
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionHuman, iPS Selection Kit
OverviewMillipore’s Human iPS Selection Kit is a quick, easy and non-invasive method to monitor the pluripotent state of live human ESC and fully reprogrammed human iPS cells using immunocytochemistry (ICC). The Human iPS Selection Kit allows live cell imaging and identification of fully reprogrammed human iPS cells from a heterogeneous population of reprogramming intermediates and enables the live selection of human iPS cells that can be further passaged and expanded for downstream applications. The selected live stained human iPS clones maintain normal human ESC morphology and can proliferate and be passaged normally. The kit consists of the following: (1) two conjugated antibodies that are known markers of human pluripotency, Tra-I-60 and SSEA-4, (2) a negative selection marker (CD13-PE) that is specific to fibroblasts and (3) a cell permeable Hoechst nuclear dye that possesses the dual function of labeling all cells and also distinguishing partially reprogrammed cells (Hoechst Bright) from fully reprogrammed cells (Hoechst Dim) (Chan et al,).
Background InformationRecent findings indicate that fully reprogrammed human iPS cells possess the following characteristics: (1) they downregulate expression of fibroblast marker, CD13 (2) they upregulate expression of pluripotent markers, SSEA-4 and TRA-1-60, (3) they silence the viral transgenes while (4) reactivating endogenous expression of Nanog and (5) they assume a HoeschstDim phenotype. These characteristics have enabled reliable identification of fully reprogrammed human iPS cells from a mixed population of partially reprogrammed cells.
References
Product Information
Components
  • Anti-SSEA-4 PE (Mouse monoclonal IgG3), Cat. No. CS204438. One vial containing 50 μg of 0.25 mg/mL purified mouse IgG antibody
  • Anti-TRA-1-60 FITC (Mouse monoclonal IgM), Cat. No. CS204460. One vial containing 50 μg of 0.5 mg/mL purified mouse IgM antibody
  • Anti-CD13 PE (Mouse monoclonal IgG1), Cat. No. CS204449. One vial containing 50 μg of 1 mg/mL purified mouse IgG antibody
  • Hoechst Nuclear Stain, Cat. No. CS204269. One vial containing 15 μL of 1 mg/mL stock solution.
Quality LevelMQ100
Applications
ApplicationThe Human iPS Selection Kit allows live cell imaging & identification of fully reprogrammed human induced pluripotent Stem cells from a heterogeneous population of reprogramming intermediates.
Key Applications
  • Immunocytochemistry
Biological Information
Species Reactivity
  • Human
Stem Cell Type
  • Human Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Maintain refrigerated at 2-8°C, protected from light in undiluted aliquots.
Packaging Information
Material Size1 kit
Material PackageSufficient for 40 tests per antibody
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
SCR502 04053252423338