Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

03-310 In Vivo SHAPE Reagent for Live Cell RNA Structure Analysis

In Vivo SHAPE Reagent contains 2-methylnicotinic acid imidazolide (NAI). This reagent has very low toxicity and permits the analysis of RNA structure in living cells using Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
03-310
0.5 mL of 2M NAI in DMSO (>97% pure)  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number03-310
Brand Family Upstate
DescriptionIn Vivo SHAPE Reagent for Live Cell RNA Structure Analysis
OverviewFeatures & Advantages
  • Ready-to-use RNase-free solution
  • Low toxicity allows use with live cells
  • High cell-permeability for rapid modification of RNA
  • Reacts with all RNA nucleotides without bias for high resolution mapping


In Vivo SHAPE Reagent is used for the determination of RNA structure. This reagent contains a highly pure form of 2-methylnicotinic acid imidazolide (NAI). This compound forms 2’-O-adducts on single stranded regions of RNA that can be detected by Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).

In contrast to other reagents used for the SHAPE method, the NAI–based In Vivo SHAPE Reagent is rapidly absorbed into cells and is formulated to have low levels of toxicity making it ideal for use with live cells. Unlike other reagents used for SHAPE analysis, In Vivo SHAPE Reagent reacts in an unbiased fashion with all four RNA nucleotides. This combination of low toxicity, rapid uptake and lack of nucleotide bias enables high resolution RNA structure analysis in a variety of living systems.
Background InformationFolding into higher-order structures allows RNAs to function inside living cells and form dynamic complexes with effector proteins. Predicting these 3D structures is challenging and therefore a number of probing techniques have been developed to study RNA folding.

Compounds such as 2-methylnicotinic acid imidazolide (NAI) contained the In Vivo SHAPE Reagent result in the formation of 2’-O-adducts. These adducts form rapidly on single stranded unpaired RNA and much more slowly on based paired (double stranded) RNA. Modified RNA can then be analyzed by primer extension. Modified nucleotides are not extended by RNA dependent DNA polymerases thus allowing sites of modification to be detected.

References
1. Wilkinson K., et al. (2006). Nature Protocols,1(3): 1610-1616
2. Spitale R., et al. (2012). Nature Chemical Biology. 9(1):18-20
References
Product Information
Components
  • 2-methylnicotinic acid imidazolide (NAI) in DMSO
HS Code3824 99 89
Formulation
Presentation0.5 mL vial containing 2M NAI (>97% pure) in DMSO solution
Quality LevelMQ100
Applications
ApplicationIn Vivo SHAPE Reagent contains 2-methylnicotinic acid imidazolide (NAI). This reagent has very low toxicity and permits the analysis of RNA structure in living cells using Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).
Key Applications
  • Reverse transcriptase assays
Biological Information
Concentration2M
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity of NAI compound is tested by HPLC.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore NAI at -20°C in a non-frost-free freezer protected from light. With proper storage (protect from light and avoid excessive freeze-thaw cycles) this product is stable for up to 6 months from the date of receipt.
Packaging Information
Material Size0.5 mL of 2M NAI in DMSO (>97% pure)
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-310 04055977113631

Documentation

In Vivo SHAPE Reagent for Live Cell RNA Structure Analysis SDS

Title

Safety Data Sheet (SDS) 

In Vivo SHAPE Reagent for Live Cell RNA Structure Analysis Certificates of Analysis

TitleLot Number
In Vivo SHAPE Reagent - 2565437 2565437
In Vivo SHAPE Reagent - 3185069 3185069
In Vivo SHAPE Reagent - 3199209 3199209
In Vivo SHAPE Reagent - 3228359 3228359
In Vivo SHAPE Reagent - 3254591 3254591
In Vivo SHAPE Reagent - 3286090 3286090
In Vivo SHAPE Reagent - 3329096 3329096
In Vivo SHAPE Reagent - 3463657 3463657
In Vivo SHAPE Reagent - 3481466 3481466
In Vivo SHAPE Reagent - 3488950 3488950
Show more