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17-10188 Sigma-AldrichLentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Brochures
Posters

Key Applications: Transfection, Immunofluorescence, Immunocytochemistry

Recommended Products

Overview

Replacement Information

Key Specifications Table

Key Applications	Detection Methods
TFX, IF, ICC	Fluorescent

Description
Catalogue Number	17-10188
Trade Name	LentiBrite Chemicon
Description	LentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor
Overview	*Read our application note in Nature Methods!* http://www.nature.com/app_notes/nmeth/2012/121007/pdf/an8620.pdf (Click Here!) Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click Here Biosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time. Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity. EMD Millipore is introducing LentiBrite™ Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis. • Pre-packaged, fluorescently-tagged with GFP & RFP • Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods • Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells •Non-disruptive towards cellular function EMD Millipore’s LentiBrite™ RFP-LC3-G120A lentiviral particles serve as a negative control alongside RFP-LC3 wild-type (catalog # 17-10143) for live cell analysis of autophagy.
Alternate Names	Microtubule-associated proteins 1A/1B light chain 3 Autophagy-related protein LC3 Autophagy-related ubiquitin-like modifier LC3 MAP1A/MAP1B light chain 3 Microtubule-associated protein 1 light chain 3
Background Information	Autophagy, a stress-induced degradative pathway, plays a role in many diseases, including cancer, neurodegeneration, and infections. Members of the LC3 family play a key role in the maturation of the autophagosome, the central organelle of autophagy. LC3 precursors are proteolytically processed to form cytosolic LC3-I. Upon initiation of autophagy, the C-terminal glycine is modified by addition of phosphatidylethanolamine (PE) to form LC3-II, which translocates to autophagosomes in a punctate distribution. DNA constructs encoding fluorescent proteins fused to LC3 are widely employed for monitoring autophagosome formation by fluorescence microscopy. A mutant form of LC3 with the C-terminal glycine changed to alanine (LC3-G120A) is unable to accept the PE modification and fails to translocate to the autophagosome upon induction of autophagy. EMD Millipore’s LentiBrite™ RFP-LC3-G120A lentiviral particles serve as a negative control alongside RFP-LC3 wild-type (catalog # 17-10143) for live cell analysis of autophagy.

References

Product Information
Components	TagRFP-LC3-G120A Lentivirus: One vial containing 25 µL of lentiviral particles at a minimum of 3 x 10E8 infectious units (IFU) per mL. For lot-specific titer information, please see lot specific “Viral Titer” in the product specifications of the datasheet. Promoter EF-1 (Elongation Factor-1) Multiplicty of Infection (MOI) MOI = Ratio of # of infectious lentiviral particles (IFU) to # of cells being infected. Typical MOI values for high transduction efficiency and signal intensity are in the range of 20-40. For this target, some cell types may require lower MOIs (e.g., HT-1080, HeLa), while others may require higher MOIs (e.g., human umbilical vein endothelial cells (HUVEC), human mesenchymal stem cells (HuMSC), U2OS). NOTE: MOI should be titrated and optimized by the end user for each cell type and lentiviral target to achieve desired transduction efficiency and signal intensity.
Detection method	Fluorescent
Quality Level	MQ100

Applications
Key Applications	Transfection Immunofluorescence Immunocytochemistry
Application Notes	Fluorescence Microscopy Imaging: (See Figure 1 in datasheet) HT-1080 cells were plated in a chamber slide and transduced with lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were either left in complete media or incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes. Cells were fixed with formaldehyde and mounted. Images were obtained by oil immersion wide-field fluorescence microscopy. The RFP-LC3 Control Mutant displays a diffuse nuclear and cytosolic distribution in both fed and starved autophagic cells (i.e., no translocation to a punctate cytoplasmic distribution as characteristic of wild-type LC3). Immunocytochemistry Comparison and Inhibitor Analysis: (See Figure 2 in datasheet) Similar to Figure 1 (see datasheet), HeLa cells were plated in a chamber slide and transduced with lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were either left in complete media, incubated for 4 hours in EBSS containing a lysosome inhibitor to induce autophagy and inhibit lysosomal degradation, or incubated as in, with the addition of 5 mM 3-methyladenine (3-MA) as an inhibitor of autophagy. 3-MA does not affect RFP-LC3 Control Mutant localization. Immunocytochemical staining (green) of the same fields of view with a monoclonal antibody against LC3A reveals a similar expression pattern to the mutant protein (red) under fed conditions. Immunostaining of starved cells displays the punctate distribution of endogenous wild-type LC3, while signal following 3-MA treatment is diminished. Hard-to-transfect Cell Types: (See Figure 3 in datasheet) Primary cell types HUVEC (top row) or HuMSC (bottom row) were plated in chamber slides and transduced with lentiviral particles at an MOI of 40 for 24 hours. Subsequent treatments for cells left in complete media or cells incubated in EBSS with lysosome inhibitor, were performed as in Figures 1A and 1B (see datasheet). For optimal fluorescent visualization, it is recommended to analyze the target expression level within 24-48 hrs after transfection/infection for optimal live cell analysis, as fluorescent intensity may dim over time, especially in difficult-to-transfect cell lines. Infected cells may be frozen down after successful transfection/infection and thawed in culture to retain positive fluorescent expression beyond 24-48 hrs. Length and intensity of fluorescent expression varies between cell lines. Higher MOIs may be required for difficult-to-transfect cell lines.

Applications

Key Applications

Transfection
Immunofluorescence
Immunocytochemistry

Application Notes

Fluorescence Microscopy Imaging:
(See Figure 1 in datasheet)
HT-1080 cells were plated in a chamber slide and transduced with lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were either left in complete media or incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes. Cells were fixed with formaldehyde and mounted. Images were obtained by oil immersion wide-field fluorescence microscopy. The RFP-LC3 Control Mutant displays a diffuse nuclear and cytosolic distribution in both fed and starved autophagic cells (i.e., no translocation to a punctate cytoplasmic distribution as characteristic of wild-type LC3).

Immunocytochemistry Comparison and Inhibitor Analysis:
(See Figure 2 in datasheet)
Similar to Figure 1 (see datasheet), HeLa cells were plated in a chamber slide and transduced with lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were either left in complete media, incubated for 4 hours in EBSS containing a lysosome inhibitor to induce autophagy and inhibit lysosomal degradation, or incubated as in, with the addition of 5 mM 3-methyladenine (3-MA) as an inhibitor of autophagy. 3-MA does not affect RFP-LC3 Control Mutant localization. Immunocytochemical staining (green) of the same fields of view with a monoclonal antibody against LC3A reveals a similar expression pattern to the mutant protein (red) under fed conditions. Immunostaining of starved cells displays the punctate distribution of endogenous wild-type LC3, while signal following 3-MA treatment is diminished.

Hard-to-transfect Cell Types:
(See Figure 3 in datasheet)
Primary cell types HUVEC (top row) or HuMSC (bottom row) were plated in chamber slides and transduced with lentiviral particles at an MOI of 40 for 24 hours. Subsequent treatments for cells left in complete media or cells incubated in EBSS with lysosome inhibitor, were performed as in Figures 1A and 1B (see datasheet).

For optimal fluorescent visualization, it is recommended to analyze the target expression level within 24-48 hrs after transfection/infection for optimal live cell analysis, as fluorescent intensity may dim over time, especially in difficult-to-transfect cell lines. Infected cells may be frozen down after successful transfection/infection and thawed in culture to retain positive fluorescent expression beyond 24-48 hrs. Length and intensity of fluorescent expression varies between cell lines. Higher MOIs may be required for difficult-to-transfect cell lines.

Biological Information
Gene Symbol	MAP1LC3 LC3
Purification Method	PEG precipitation
UniProt Number	Q9GZQ8 Q9H492

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by transduction of HT-1080 cells and fluorescent imaging performed for assessment of transduction efficiency.
Usage Statement	This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by transduction of HT-1080 cells and fluorescent imaging performed for assessment of transduction efficiency.

Usage Statement

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Storage and Handling Lentivirus is stable for at least 4 months from date of receipt when stored at -80°C. After first thaw, place immediately on ice and freeze in working aliquots at -80°C. Frozen aliquots may be stored for at least 2 months. Further freeze/thaws may result in decreased virus titer and transduction efficiency. IMPORTANT SAFETY NOTE Replication-defective lentiviral vectors, such as the 3rd Generation vector provided in this product, are not known to cause any diseases in humans or animals. However, lentiviruses can integrate into the host cell genome and thus pose some risk of insertional mutagenesis. Material is a Risk Group 2 and should be handled under BSL2 controls. A detailed discussion of biosafety of lentiviral vectors is provided in Pauwels, K. et al. (2009). State-of-the-art lentiviral vectors for research use: Risk assessment and biosafety recommendations. Curr. Gene Ther. 9: 459-474.

Storage and Shipping Information

Storage Conditions

Storage and Handling
Lentivirus is stable for at least 4 months from date of receipt when stored at -80°C. After first thaw, place immediately on ice and freeze in working aliquots at -80°C. Frozen aliquots may be stored for at least 2 months. Further freeze/thaws may result in decreased virus titer and transduction efficiency.

IMPORTANT SAFETY NOTE
Replication-defective lentiviral vectors, such as the 3rd Generation vector provided in this product, are not known to cause any diseases in humans or animals. However, lentiviruses can integrate into the host cell genome and thus pose some risk of insertional mutagenesis. Material is a Risk Group 2 and should be handled under BSL2 controls. A detailed discussion of biosafety of lentiviral vectors is provided in Pauwels, K. et al. (2009). State-of-the-art lentiviral vectors for research use: Risk assessment and biosafety recommendations. Curr. Gene Ther. 9: 459-474.

Packaging Information
Material Size	1 vial (minimum of 3 x 10E8 IFU/mL)

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
17-10188	04053252549236

Documentation

LentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor SDS

Title
Safety Data Sheet (SDS)

LentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor Certificates of Analysis

Title	Lot Number
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor - 2055469	2055469
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor - 2201532	2201532
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor - 2224455	2224455
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor - Q1988423	Q1988423
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor - 2475931	2475931
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor - 3560020	3560020
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor - 3988510	3988510
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor Packaged Lentiviral Particles	3136397
LentiBrite RFP-LC3 Control Mutant Lentiviral Biosensor Packaged Lentiviral Particles	3047047

Brochure

Title
Hallmarks of Aging

Posters

Title
Poster: Autophagy Signaling

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17-10188 Sigma-AldrichLentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor

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Overview

Key Specifications Table

Documentation

LentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor SDS

LentiBrite™ RFP-LC3 Control Mutant Lentiviral Biosensor Certificates of Analysis

Brochure

Posters

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