Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

101287 Löffler's methylene blue solution

for microscopy

Löffler's methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Löfflere'smethylene blue solution, Methylene blue solution acc. to Löffler

View Products on Sigmaaldrich.com
101287
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
1012870100 Plastic bottle 100 ml
1012870500 Plastic bottle 500 ml
1012872500 Plastic bottle 2.5 l
Description
Catalogue Number101287
SynonymsLöfflere'smethylene blue solution, Methylene blue solution acc. to Löffler
DescriptionLöffler's methylene blue solution
OverviewLöffler’s methylene blue solution - for microscopy, is a ready-to-use-solution and is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use methylene blue is used for counterstaining.
Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material.
The 500 ml bottle is sufficient for approx. 250 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor microscopy
Biological Information
Physicochemical Information
Density0.97 g/cm3 (20 °C)
Flash point40 °C
pH value7.0 - 7.5 (H₂O, 20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H226: Flammable liquid and vapour.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P240: Ground and bond container and receiving equipment.
P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
P242: Use non-sparking tools.
P243: Take action to prevent static discharges.
Signal WordWarning
Storage class3 Flammable liquids
WGKWGK 1 slightly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerflammable
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1993 , 3, III
Declaration (transport by air) IATA-DGRUN 1993 , 3, III
Declaration (transport by sea) IMDG-CodeUN 1993 , 3, III
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
1012870100 04022536019024
1012870500 04022536019048
1012872500 04022536019062