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109261 Lugol's solution (diluted iodine-potassium iodide solution)

for the Gram staining method

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Iodine-potassium iodide solution

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109261
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
1092611000 Glass bottle 1 l
1092612500 Glass bottle 2.5 l
Description
Catalogue Number109261
SynonymsIodine-potassium iodide solution
DescriptionLugol's solution (diluted iodine-potassium iodide solution)
OverviewLugol’s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Cat.no. 109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step (Gram's decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Cat.no. 109217) and will then appear pink to red.
Lugol's solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor the Gram staining method
Biological Information
Physicochemical Information
Boiling point100 °C (1013 hPa)
Density1.01 g/cm3 (20 °C)
pH value3.5 (H₂O, 20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 2 obviously hazardous to water
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Suitability for microscopypasses test
gram-positve microorganismsblue-violet
gram-negative microorganismspink to red
Global Trade Item Number
Catalog Number GTIN
1092611000 04022536112701
1092612500 04022536557410

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Alternative Products

Catalog Number Description
100567 Lugol's solution

Related Products

Catalog Number Description
100567 Lugol's solution

Accessories (Primary)

Catalog Number Description
109217 Gram's safranine solution
110218 Gram's decolorizing solution

Accessories (Secondary)

Catalog Number Description
109217 Gram's safranine solution

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Categories

IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining