Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

14-515 MAP Kinase 1/Erk1 Protein, inactive, 50 µg

Inactive, N-terminal GST-tagged, recombinant, full-length, human MAP Kinase 1/Erk1, for use in Kinase Assays.

MAP Kinase 1/Erk1 Protein, inactive, 50 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
14-515
50 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-515
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMAP Kinase 1/Erk1 Protein, inactive, 50 µg
OverviewN-terminal GST-tagged, recombinant, full-length, human MAP Kinase 1/Erk1
References
Product Information
Quality LevelMQ100
Applications
ApplicationInactive, N-terminal GST-tagged, recombinant, full-length, human MAP Kinase 1/Erk1, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Gene Symbol
  • MAPK1
  • p40
  • PRKM2
  • MAPK2
  • PRKM1
  • p42-MAPK
  • p38
  • p41
  • ERK
  • ERK-2
  • ERT1
  • P42MAPK
  • p41mapk
  • ERK2
Protein TargetMAPK1
Purification MethodGlutathione-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight70kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP substrate in vitro
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20°C
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-515 04053252731983

Related Products & Applications

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Erk (MAP) Kinase Antibodies

Millipore’s Erk Antibodies demonstrate specificity against Erk kinases, p44 and p42 MAP kinases. See below for related products, including proteins & kits based on the expertise of Upstate & Chemicon.
Learn More >>

MAPK Antibodies, Proteins, Inhibitors, & Assays

Offering hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases