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454849 MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: ERK1, MAPK3, p44MAPK

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454849
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
454849-2.5UG Plastic ampoule 2.5 μg
Description
OverviewRecombinant, human MAP Kinase 1 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Catalogue Number454849
Brand Family Calbiochem®
SynonymsERK1, MAPK3, p44MAPK
References
ReferencesCharest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥6000 units/mg protein
Physicochemical Information
ContaminantsProteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
454849-2.5UG 04055977204209

Documentation

MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
454849

References

Reference overview
Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.

Brochure

Title
MAPK Pathway Poster ( 750 KB )
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2008 RFH
SynonymsERK1, MAPK3, p44MAPK
DescriptionRecombinant, human MAP Kinase 1 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Purity≥95% by SDS-PAGE
ContaminantsProteases: none detected
Specific activity≥6000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesCharest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.