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14-337M MAPKAP Kinase 2 Protein, active, 250 µg

Active, for use in Kinase Assays.

MAPKAP Kinase 2 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-337M
250 µg  Also available in 10 μg size (cat#14-337) and in bulk (cat# 14-337-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-337M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMAPKAP Kinase 2 Protein, active, 250 µg
Background InformationRecombinant human MAPKAP Kinase 2, residues 46-400, A399G, containing an N-terminal GST tag.

MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis.
The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman MAPKAP Kinase 2 expressed in E. coli.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Gene Symbol
  • MAPKAPK2
  • MK2
  • MAPKAPK-2
Protein TargetMAPKAP-K2
Purification MethodGSH-agarose & Q-Sepharose™ FF
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
SIZE: 400 amino acids; 45568 Da
SUBUNIT: Interacts with PHC2.
TISSUE SPECIFICITY: Expressed in all tissues examined.
PTM: Phosphorylated and activated by MAP kinase.
SIMILARITY: SwissProt: P49137 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
Molecular Weight70.2kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of MAPKAP Kinase 2 Substrate Peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-337) and in bulk (cat# 14-337-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-337M 04053252324253

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-337 MAPKAP Kinase 2 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases