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13-173 MBP, recombinant

MBP, recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays UniProt Number: P02686 Entrez Gene Number: NM_001025094.1, NM_001025098.1, NM_001025081.1, NM_001025090.1, NM_001025092.1, NM_001025100.1, NM_001025101.1, NM_002385.2
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13-173
500 µg  
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Description
Catalogue Number13-173
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMBP, recombinant
Alternate Names
  • myelin basic protein
References
Product Information
Presentation500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
Quality LevelMQ100
Applications
Key Applications
  • Enzyme Assays
Biological Information
Concentration1 mg/mL
PurityPurity information is lot specific, please consult the certificate of analysis
SourceRecombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.
Specific Activity<b>Non-radioactive kinase assay:</b> This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
Gene Symbol
  • MBP
  • MGC99675
Purification MethodProBond Resin chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
SIZE: 304 amino acids; 33117 Da
SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family.
Molecular Weight~47 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by kinase assay
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
13-173 04053252477201

Documentation

MBP, recombinant SDS

Title

Safety Data Sheet (SDS) 

MBP, recombinant Certificates of Analysis

TitleLot Number
MBP, recombinant (recombinant protein expressed in E. coli) 2871336
MBP, recombinant (recombinant protein expressed in E. coli) - 2066700 2066700
MBP, recombinant (recombinant protein expressed in E. coli) - 2234162 2234162
MBP, recombinant (recombinant protein expressed in E. coli) - 3290291 3290291
MBP, recombinant - 3704569 3704569
MBP, recombinant - 4058593 4058593
MBP, recombinant - JBC1865913 JBC1865913
MBP, recombinant - R0703D0001 R0703D0001
MBP, recombinant -2621524 2621524
MBP, recombinant -2751275 2751275

Data Sheet

Title
MBP, recombinant

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Categories

Life Science Research > Proteins and Enzymes > Other Proteins