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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

HCY4MG-64K-PX20 MilliporeMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV, Premixed 20 Plex Assay

The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.

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Overview

Replacement Information

Description
Catalogue Number	HCY4MG-64K-PX20
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV, Premixed 20 Plex Assay
Overview	EMD Millipore’s MILLIPLEX® MAP Cytokine/Chemokine Magnetic Bead Panel IV Premix is a 20-plex premixed kit to be used for the simultaneous quantification of all of the following analytes in serum, plasma or culture supernatant samples: BAFF/Blys, BRAK/CSCL14, CCL28, CXCL16, HMGB1, IFNβ, IL-14, IL-19, IL-24, IL-28B, IL-32α, IL-34, IL-35, IL-36β, IL-37, IL-38, MIP-4/PARC/CCL18, MPIF-1/CL23 and YKL40/CHI3L1. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: • Quality controls (QCs) provided to qualify assay performance • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency • Optimized serum matrix to mimic native analyte environment • Detection antibody cocktails designed to yield consistent analyte profiles within panel EMD Millipore’s MILLIPLEX® MAP Human Cytokine/Chemokine Magnetic Bead Panel IV is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background Information	Inflammation and immune cell response are key to maintaining homeostasis. The more we understand mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease: certainly immunological diseases, such as allergic reactions, asthma, infectious disease and an ever-growing number of conditions under the autoimmune umbrella, but also neurodegenerative disease, metabolic and cardiovascular disease and even cancer. The ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX ® MAP panels, based on the Luminex xMAP® technology, to establish disease profiles that can be used for screening, diagnosis and response to treatment is critical for understanding the pathogenesis of these diseases.

References

Product Information
Detection method	Luminex xMAP
Configuration	Premixed
Precision, %	Intra-assay: 10 Inter-assay:15-25
Panel Type	MAGNETIC Cytokines/Chemokines

Applications
Application	The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended. • A maximum of 25 μL per well of 1:4 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. Please note that HMGB1 can be measured in serum and plasma samples only.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Human
Analytes Available	BAFF/Blys BRAK/CXCL14 CCL28 CXCL16 HCC-4/CCL16 HMGB1 IFNβ IL-14 IL-19 IL-24 IL-28B IL-36β/IL-1F8 IL-32α IL-34 IL-35 IL-37/IL-1F7 IL-38/IL-1F10 MIP-4 MPIF-1/CCL23 YKL40
Cross Reactivity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy	BAFF/Blys: 101% BRAK/CSCL14: 101% CCL28: 91% CXCL16: 103% HCC-4/CCL16: 110% HMGB1: 104% IFNβ: 97% IL-14: 104% IL-19: 106% IL-24: 99% IL-28B: 101% IL-32α: 89% IL-34: 110% IL-35: 95% IL-36β: 98% IL-37: 99% IL-38: 104% MIP-4/PARC/CCL18: 101% MPIF-1/CL23: 102% YKL40/CHI3L1: 100%

Physicochemical Information
Sensitivity	MPIF-1: 7.8 pg/mL BRAK: 607 pg/mL CXCL16: 4.7 pg/mL HCC-4: 28.4 pg/mL MIP-4/PARC: 6.6 pg/mL IL-34: 121.5 pg/mL IL-24: 0.8 ng/mL IL-35: 0.8 ng/mL IL-37/IL-1F7: 5.2 pg/mL IL-19: 248.2 pg/mL CCL28: 100.8 pg/mL HMGB1/HMG1: 10.5 ng/mL IFNβ: 375.6 pg/mL IL-38: 33.2 pg/mL IL-28B/IFN-λ3: 17.2 pg/mL BAFF/BLyS: 0.9 ng/mL IL-14/α-Taxilin: 27.7 pg/mL IL-36β: 3.5pg/mL IL-32α: 32.1 pg/mL YKL40/CHI3L1: 0.30 ng/mL
Standard Curve Range	BAFF/Blys: 0.44-400 ng/mL BRAK/CXCL14: 488-500,000 pg/mL CCL28: 98-100,000 pg/mL CXCL16: 3-3,000 pg/mL HMGB1: 2.0-2,000 ng/mL IFNβ: 488 – 500,000 pg/mL IL-14: 24-25,000 pg/mL IL-19: 244-250,000 pg/mL IL-24: 0.7-750 ng/mL IL-28B: 29-30,000 pg/mL IL-32α: 39-40,000 pg/mL IL-34: 98-100,000 pg/mL IL-35: 0.8-800 ng/mL IL-36β: 2- 2,000 pg/mL IL-37: 5 – 5,000 pg/mL IL-38: 49-50,000 pg/mL MIP-4/PARC/CCL18: 5-5,000 pg/mL MPIF-1/CL23: 10-10,000 pg/mL YKL40/CHI3L1: 0.1-100 ng/mL

Physicochemical Information

Sensitivity

MPIF-1: 7.8 pg/mL
BRAK: 607 pg/mL
CXCL16: 4.7 pg/mL
HCC-4: 28.4 pg/mL
MIP-4/PARC: 6.6 pg/mL
IL-34: 121.5 pg/mL
IL-24: 0.8 ng/mL
IL-35: 0.8 ng/mL
IL-37/IL-1F7: 5.2 pg/mL
IL-19: 248.2 pg/mL
CCL28: 100.8 pg/mL
HMGB1/HMG1: 10.5 ng/mL
IFNβ: 375.6 pg/mL
IL-38: 33.2 pg/mL
IL-28B/IFN-λ3: 17.2 pg/mL
BAFF/BLyS: 0.9 ng/mL
IL-14/α-Taxilin: 27.7 pg/mL
IL-36β: 3.5pg/mL
IL-32α: 32.1 pg/mL
YKL40/CHI3L1: 0.30 ng/mL

Standard Curve Range

BAFF/Blys: 0.44-400 ng/mL
BRAK/CXCL14: 488-500,000 pg/mL
CCL28: 98-100,000 pg/mL
CXCL16: 3-3,000 pg/mL
HMGB1: 2.0-2,000 ng/mL
IFNβ: 488 – 500,000 pg/mL
IL-14: 24-25,000 pg/mL
IL-19: 244-250,000 pg/mL
IL-24: 0.7-750 ng/mL
IL-28B: 29-30,000 pg/mL
IL-32α: 39-40,000 pg/mL
IL-34: 98-100,000 pg/mL
IL-35: 0.8-800 ng/mL
IL-36β: 2- 2,000 pg/mL
IL-37: 5 – 5,000 pg/mL
IL-38: 49-50,000 pg/mL
MIP-4/PARC/CCL18: 5-5,000 pg/mL
MPIF-1/CL23: 10-10,000 pg/mL
YKL40/CHI3L1: 0.1-100 ng/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate
Material Package	96 well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
HCY4MG-64K-PX20	04061841042662

Documentation

Protocols

Title
Protocol- HCYP4MAG-64K

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HCY4MG-64K-PX20 MilliporeMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel IV, Premixed 20 Plex Assay

The Human Inflammation / Immunology Bead-Based Multiplex Cytokine/Chemokine Premix Assay kit IV, using the Luminex xMAP technology, enables the simultaneous analysis of 20 cytokine and chemokine biomarkers in human serum, plasma and cell culture samples.

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Overview

Documentation

Protocols

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