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HIL18MAG-66K MILLIPLEX MAP Human IL-18 Singleplex Magnetic Bead Kit

This kit is validated for serum, plasma or tissue/cell supernatant samples, and for use as a single protein quantification assay or combined with
• Human Cytokine/Chemokine Panel 1 (HCYTOMAG-60K)
• Human Cytokine/Chemokine Panel 2 (HCYP2MAG-62K except CTACK/CCL27)
• Human Th17 Panel (HTH17MAG-14K)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology, Immune Response
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HIL18MAG-66K
96-well plate  
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IL-18 HMplexLuminex xMAP
Description
Catalogue NumberHIL18MAG-66K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human IL-18 Singleplex Magnetic Bead Kit
OverviewEMD Millipore’s MILLIPLEX® MAP Human IL-18 Singleplex Kit is validated for detection in serum, plasma or tissue/cell supernatant samples, and for use as follows:
• On its own as a single protein quantification assay, Cat. No. HIL18MAG-66K (the standard is calibrated to the WHO standard)
• Combined with MILLIPLEX® MAP Human Cytokine/Chemokine Panel 1, Cat. No. HCYTOMAG-60K (or the corresponding premix or bulk format kits)
• Combined with MILLIPLEX® MAP Human Cytokine/Chemokine Panel 2, Cat. No. HCYP2MAG-62K (with the exception of the CTACK/CCL27 assay, due to bead region overlap)
• Combined with MILLIPLEX® MAP Human Th17 Panel, Cat. No. HTH17MAG-14K (or the corresponding premix or bulk format kits)
Background InformationInterleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a member of the IL-1 cytokine superfamily and acts as a pleiotropic cytokine that modulates both innate and acquired immune responses. Disrupted regulation of IL-18 bioactivity is linked to multiple types of diseases including chronic inflammatory diseases, autoimmune diseases, a variety of cancers, and infectious diseases. It also has been implicated to play a role in emphysema, myocardial function, cardiovascular disease, metabolic syndrome, acute kidney injury and neurodegeneration. Our MILLIPLEX® MAP Human IL-18 Singleplex Magnetic Bead Kit is a single analyte kit to be used for the quantification of IL-18 in serum, plasma, and tissue/cell supernatant samples. The Human IL-18 Singleplex standard is calibrated to the WHO standard.
References
Product Information
Detection methodLuminex xMAP
ConfigurationSingle Plex
Precision, %
  • Intra-assay:< 5% CV, Inter-assay: < 10% CV (in single analyte assay)
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
Application This kit is validated for serum, plasma or tissue/cell supernatant samples, and for use as a single protein quantification assay or combined with
• Human Cytokine/Chemokine Panel 1 (HCYTOMAG-60K)
• Human Cytokine/Chemokine Panel 2 (HCYP2MAG-62K except CTACK/CCL27)
• Human Th17 Panel (HTH17MAG-14K)
Key Applications
  • Multiplexing
Application Notes• A maximum of 25 μL per well neat serum or plasma can be used. Tissue culture or other media may be run neat or appropriately diluted in control medium.
• As single analyte assay, this assay may be run as a 2 hour or overnight incubation assay. If combining with one of the other indicated panels, please follow the protocol for that particular panel.
• When running this assay as a single analyte assay, follow the protocol directions and use the reagents that come in the HIL18MAG-66K kit.
• When combining the IL-18 analyte with one of the larger panels, use the reagents provided in the other kit. Carefully reference both protocols to ensure proper procedures are followed, particularly for the following sections:
o “PREPARATION OF REAGENTS FOR IMMUNOASSAY: HUMAN IL-18 WITH OTHER KITS”
- Note: Use the serum matrix (if needed) provided with the larger panel
o Follow the “IMMUNOASSAY PROCEDURE” for the larger panel
• If comparing single analyte IL-18 sample values with IL-18 combined with one of the possiible kitss, you must run a curve to determine sample correlation and slope. Differences in sample values are expected due to differences in serum matrix, but should highly correlate.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • IL-18
Cross ReactivityCross-reactivity is non-detectable or negligible between the antibodies in this kit and the antibodies of the panels it can be combined into:
• Human Cytokine/Chemokine Panel 1 (HCYTOMAG-60K or the corresponding premix or bulk format kits)
• Human Cytokine/Chemokine Panel 2 (HCYP2MAG-62K (with the exception of the CTACK/CCL27 assay, due to bead region overlap))
• Human Th17 Panel (HTH17MAG-14K or the corresponding premix or bulk format kits)
Accuracy
  • 99% (in single analyte assay)
Physicochemical Information
Sensitivity
  • MinDC + 2SD (pg/mL)
  • 2.17 (in single analyte assay)
Standard Curve Range
  • 1.6 - 25,000 pg/mL (in single analyte assay)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HIL18MAG-66K 04054839535499