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HMYOMAG-56K MILLIPLEX MAP Human Myokine Magnetic Bead Panel

The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Obesity, Metabolic Disorders, Inflammation & Autoimmune Mechanisms
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HMYOMAG-56K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Apelin BDNF Erythropoietin (EPO) FABP3 FGF-21 Fractalkine/CX3CL1 Follistatin-like Protein 1 (FSTL1) IL-6 IL-15 Irisin LIF Myostatin/GDF8 Oncostatin M (OSM) Osteocrin/Musclin Osteonectin (SPARC) HMplexLuminex xMAP
Description
Catalogue NumberHMYOMAG-56K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Myokine Magnetic Bead Panel
OverviewEMD Millipore’s MILLIPLEX® MAP Human Myokine Magnetic Bead Panel 15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum and plasma samples and in cell/tissue supernatants: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1).

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


EMD Millipore’s MILLIPLEX® MAP Human Myokine Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationMuscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only can act in autocrine and/or paracrine manner in regulation of skeletal muscle growth, they can also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations to the exercise-induced metabolic changes in liver and adipose tissue as well as the profound changes in immune and neuron systems.

The MILLIPLEX® MAP Human Myokine Magnetic Bead Panel enables you to focus on the relevance and therapeutic potential of Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1) in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Apelin: Intra-assay <10% CV Inter-assay <15%
  • BDNF: Intra-assay <10% CV Inter-assay <15%
  • Erythropoietin (EPO): Intra-assay <10% CV Inter-assay <15%
  • FABP3: Intra-assay <10% CV Inter-assay <15%
  • FGF-21: Intra-assay <10% CV Inter-assay <15%
  • Fractalkine/CX3CL1: Intra-assay <10% CV Inter-assay <15%
  • Follistatin-like Protein 1 (FSTL1): Intra-assay <10% CV Inter-assay <15%
  • IL-6: Intra-assay <10% CV Inter-assay <15%
  • IL-15: Intra-assay <10% CV Inter-assay <15%
  • Irisin: Intra-assay <10% CV Inter-assay <15%
  • LIF: Intra-assay <10% CV Inter-assay <15%
  • Myostatin/GDF8: Intra-assay <10% CV Inter-assay <15%
  • Oncostatin M (OSM): Intra-assay <10% CV Inter-assay <15%
  • Osteocrin/Musclin: Intra-assay <10% CV Inter-assay <15%
  • Osteonectin (SPARC): Intra-assay <10% CV Inter-assay <20%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationThe Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.
Key Applications
  • Multiplexing
Application NotesAlthough a two hour sample incubation may be used, for optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Apelin
  • BDNF
  • Erythropoietin (EPO)
  • FABP3
  • FGF-21
  • Fractalkine/CX3CL1
  • Follistatin-like Protein 1 (FSTL1)
  • IL-6
  • IL-15
  • Irisin
  • LIF
  • Myostatin/GDF8
  • Oncostatin M (OSM)
  • Osteocrin/Musclin
  • Osteonectin (SPARC)
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • Apelin 100%
  • BDNF 97%
  • EPO 100%
  • FABP3 79%
  • FGF21 98%
  • Fractalkine 97%
  • FSTL-1 96%
  • IL-6 82%
  • IL-15 92%
  • Irisin 96%
  • LIF 97%
  • Myostatin/GDF8 94%
  • Oncostatin M (OSM) 98%
  • Osteocrin/Musclin 97%
  • Osteonectin (SPARC) 96%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • Apelin: 64 pg/mL
  • BDNF: 9 pg/mL
  • Erythropoietin (EPO): 815 pg/mL
  • FABP3: 9.8 pg/mL
  • FGF-21: 14 pg/mL
  • Fractalkine/CX3CL1: 40 pg/mL
  • Follistatin-like Protein 1 (FSTL1): 737 pg/mL
  • IL-6: 0.9 pg/mL
  • IL-15: 5 pg/mL
  • Irisin: 281 pg/mL
  • LIF: 4 pg/mL
  • Myostatin/GDF8: 259 pg/mL
  • Oncostatin M (OSM): 6 pg/mL
  • Osteocrin/Musclin: 59 pg/mL
  • Osteonectin (SPARC): 7.5 ng/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • Apelin: 122 - 500,000 pg/mL
  • BDNF: 2 - 10,000 pg/mL
  • Erythropoietin (EPO): 244 - 1,000,000 pg/mL
  • FABP3: 24 - 100,000 pg/mL
  • FGF-21: 5 - 20,000 pg/mL
  • Fractalkine/CX3CL1: 49 - 200,000 pg/mL
  • Follistatin-like Protein 1 (FSTL1): 488 - 2,000,000 pg/mL
  • IL-6: 0.6 - 2,400 pg/mL
  • IL-15: 2 - 10,000 pg/mL
  • Irisin: 244 - 1,000,000 pg/mL
  • LIF: 10 - 40,000 pg/mL
  • Myostatin/GDF8: 488 - 2,000,000 pg/mL
  • Oncostatin M (OSM): 2 -6,000 pg/mL
  • Osteocrin/Musclin: 49 - 200,000 pg/mL
  • Osteonectin (SPARC): 7 - 30,000 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HMYOMAG-56K 04054839442889

Documentation

Protocols

Title
Protocol- HMYOMAG-56K

MILLIPLEX MAP Human Myokine Magnetic Bead Panel SDS

Title

Safety Data Sheet (SDS) 

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HMYOMAG-56K xPonent Protocol Template Version 3.1
HMYOMAG-56K xPonent Protocol Template Version Magpix
HMYOMAG-56K xPonent Standard Template Version 3.1
HMYOMAG-56K xPonent Standard Template Version Magpix
Quality Control Range Sheet- HMY-109 and HMY-209
Quality Control Ranges: MILLIPLEX® MAP Human Myokine Magnetic Bead Panel

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Categories

Life Science Research > Antibodies and Assays > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits
Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits
Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Other Inhibitors of Biological Interest