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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MCKP1-110K-PX28 MilliporeMILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1

The Mouse Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 28 immune checkpoint proteins in mouse serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples.

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Description
Catalogue Number	MCKP1-110K-PX28
Trade Name	MILLIPLEX
Description	MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1
Overview	EMD Millipore’s MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1 is a 28-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples: 4-1BBL/TNFSF9, 5’-NT/CD73, B7-H2/ICOSL, B7-H3/CD276, BCA-1/CXCL-13, BTLA, CD137/4-1BB, CD226/DNAM-1, CD25/IL-2Rα, CD27, CD40, CD80/B7-1, CTLA-4/CD152, E-cadherin, Galectin-1, Galectin-3, GITR, Granzyme B, HVEM, IFNγ, IL-10, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2, and TNFα.
Background Information	Immune checkpoint proteins are key regulators of the immune system and drug targets of cancer immunotherapy. Immune checkpoint molecules have been found on the cell surface of immune cells and tumor cells as activating receptors or inhibitory receptors. Immune checkpoint inhibitors, such as blocking antibodies to cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), programmed cell death protein-1 (PD-1) and programmed cell death ligand 1 (PD-L1), have demonstrated significant efficacy in the treatment of a variety of cancers. Recent studies have also shown many immune checkpoint proteins can undergo alternative splicing or proteolytic cleavage to produce soluble checkpoint proteins. The expression levels of soluble and cell-surface immune checkpoint proteins in the circulation and in tumor-microenvironment (TME) may be used as putative immune-based biomarkers for cancer research and biomarker discovery in immunological diseases.

References

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Inter-assay: <20 Intra-assay: <10
Panel Type	MAGNETIC Circulating Cancer
Quality Level	MQ200

Applications
Application	The Mouse Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 28 immune checkpoint proteins in mouse serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples.
Key Applications	Multiplexing
Application Notes	This is an overnight incubation assay (16-18 hours at 2-8°C). A maximum of 25 μL per well 1:2 diluted serum or plasma can be used. Tissue culture samples may be run neat or appropriately diluted in control medium. Note: Best results using cell/tissue lysate samples may require diluting in assay buffer while testing similarly diluted lysis buffer for matrix effects.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Mouse
Analytes Available	4-1BBL/TNFSF9 B7-H2/ICOSL B7-H3/CD276 5'-NT/CD73 CD137/4-1BB CD226/DNAM-1 E-Cadherin BCA-1 BTLA CD27 CD80/B7-1 CTLA-4 CD40 Galectin-1 (GAL1) Galectin-3 GITR Granzyme B HVEM IFNγ IL-10 LAG-3 PD-1 PD-L1 PD-L2 TIM-3 TLR-2 TNFα CD25/IL-2Rα
Cross Reactivity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy	BTLA: 111% 4-1BBL/TNFSF9: 105% CD137/4-1BB: 107% B7-H2/ICOSL: 110% B7-H3/CD276: 95% 5’-NT/CD73: 106% BCA-1/CXCL-13: 105% CD226/DNAM-1: 110% CD25/IL-2Rα: 105% CD27: 107% CD40: 109% CD80/B7-1: 103% CTLA-4/CD152: 104% E-Cadherin: 108% Galectin-1: 101% Galectin-3: 108% GITR: 109% Granzyme B: 94% HVEM: 108% IFNγ: 103% IL-10: 105% LAG-3: 105% PD-1 : 106% PD-L1: 96% PD-L2: 104% TIM-3: 106% TLR-2: 104% TNFα: 106%

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitives for individual analytes.
Standard Curve Range	4-1BBL/TNFSF9: 14 – 10,000 pg/mL 5’-NT/CD73: 137 – 100,000 pg/mL B7-H2/ICOSL: 27 – 20,000 pg/mL B7-H3/CD276: 3.4 – 2,500 ng/mL BCA-1/CXCL-13: 27 – 20,000 pg/mL BTLA: 21 – 15,000 pg/mL CD137/4-1BB: 5.5 – 4,000 pg/mL CD226/DNAM-1: 14 – 10,000 pg/mL CD25/IL-2Rα: 2.7 – 2,000 pg/mL CD27: 55 – 40,000 pg/mL CD40: 14 – 10,000 pg/mL CD80/B7-1: 34.3 – 25,000 pg/mL CTLA-4/CD152: 5.5 – 4,000 pg/mL E-Cadherin: 342 – 250,000 pg/mL Galectin-1: 3.4 – 2,500 ng/mL Galectin-3: 41 – 30,000 pg/mL GITR: 41 – 30,000 pg/mL Granzyme B: 8.2 – 6,000 pg/mL HVEM: 1.4 – 1,000 pg/mL IFNγ: 2.1 – 1,500 pg/mL IL-10: 11 – 8,000 pg/mL LAG-3: 4.12 – 300,000 pg/mL PD-1: 55 – 40,000 pg/mL PD-L1: 14 – 10,000 pg/mL PD-L2: 6.9 - 5,000 pg/mL TIM-3: 5.5 - 4,000 pg/mL TLR-2: 412 - 3,000 pg/mL TNFα: 4.1 - 3,000 pg/mL

Physicochemical Information

Sensitivity

Refer to kit protocol for sensitives for individual analytes.

Standard Curve Range

4-1BBL/TNFSF9: 14 – 10,000 pg/mL
5’-NT/CD73: 137 – 100,000 pg/mL
B7-H2/ICOSL: 27 – 20,000 pg/mL
B7-H3/CD276: 3.4 – 2,500 ng/mL
BCA-1/CXCL-13: 27 – 20,000 pg/mL
BTLA: 21 – 15,000 pg/mL
CD137/4-1BB: 5.5 – 4,000 pg/mL
CD226/DNAM-1: 14 – 10,000 pg/mL
CD25/IL-2Rα: 2.7 – 2,000 pg/mL
CD27: 55 – 40,000 pg/mL
CD40: 14 – 10,000 pg/mL
CD80/B7-1: 34.3 – 25,000 pg/mL
CTLA-4/CD152: 5.5 – 4,000 pg/mL
E-Cadherin: 342 – 250,000 pg/mL
Galectin-1: 3.4 – 2,500 ng/mL
Galectin-3: 41 – 30,000 pg/mL
GITR: 41 – 30,000 pg/mL
Granzyme B: 8.2 – 6,000 pg/mL
HVEM: 1.4 – 1,000 pg/mL
IFNγ: 2.1 – 1,500 pg/mL
IL-10: 11 – 8,000 pg/mL
LAG-3: 4.12 – 300,000 pg/mL
PD-1: 55 – 40,000 pg/mL
PD-L1: 14 – 10,000 pg/mL
PD-L2: 6.9 - 5,000 pg/mL
TIM-3: 5.5 - 4,000 pg/mL
TLR-2: 412 - 3,000 pg/mL
TNFα: 4.1 - 3,000 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate
Material Package	96 well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
MCKP1-110K-PX28	04065267298719

Documentation

Protocols

Title
Protocol- MCKP1-110K\|MCKP1-110K-PX28\|MCKP1-110K-PBK28

MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1 SDS

Title
Safety Data Sheet (SDS)

Technical Info

Title
QC Range Sheet MCKP1-110K
Quality Control Range Sheet- MCKP1-102 and MCKP1-202

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MCKP1-110K-PX28 MilliporeMILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1

The Mouse Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 28 immune checkpoint proteins in mouse serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples.

Recommended Products

Overview

Documentation

Protocols

MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1 SDS

Technical Info

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