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48-655MAG MILLIPLEX® Protein Translation Magnetic Bead 6-Plex Kit - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
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48-655MAG
96-well plate  96-well plate
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
eIF-4G (Ser1108) elF-4E (Ser209) elF-4B (Ser422) elF-2a (Ser51) 4E-BP1 (Total) 4E-BP1 (Thr37/46) H, M, RMplexLuminex xMAP
Description
Catalogue Number48-655MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Protein Translation Magnetic Bead 6-Plex Kit - Cell Signaling Multiplex Assay
OverviewEach MILLIPLEX® cell signaling kit includes:
• Stimulated and unstimulated cell lysates provided to qualify assay performance
• Premixed magnetic beads to capture analytes of interest
• Optimized detection antibody cocktails designed to yield consistent analyte profiles within a panel

The MILLIPLEX® Protein Translation 6-plex Magnetic Bead kit is used to detect changes in phosphorylated eIF2a (Ser51), eIF-4B (Ser422), eIF-4E (Ser209), eIF-4G (Ser1108) and 4E-BP1 (Thr37/46), as well as total protein levels of 4E-BP1 in cell lysates using the Luminex® system. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.
Background InformationProtein synthesis is required for cellular growth and development. Normal cells grow in a controlled manner in response to environmental and developmental cues. However, cancer cells can reprogram cellular metabolism, favoring uncontrolled growth and survival. This can be achieved by altering signaling pathways that control cellular processes such as protein synthesis.

The phosphorylation state of proteins involved in translation initiation is a limiting factor that regulates the formation or activity of translational complexes. In cancer cells, hyper-activated signaling pathways influence translation, allowing uncontrolled growth and survival. In addition, several components of translation initiation have been found to be mutated, post-translationally modified, or differentially expressed, and thus have been shown to act as oncogenes.

Translational alterations can increase the overall rate of protein synthesis as well as activate regulatory mechanisms leading to the translation of specific messenger RNAs for proteins that promote cancer progression and survival.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • eIF-4G (Ser1108)
  • elF-4E (Ser209)
  • elF-4B (Ser422)
  • elF-2a (Ser51)
  • 4E-BP1 (Total)
  • 4E-BP1 (Thr37/46)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
48-655MAG 04054839156960