Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

03-312 Magna ChIRP Human HOTAIR lncRNA Probe Set

The Magna ChIRP Human HOTAIR lncRNA Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of Human lncRNA HOTAIR.

View Products on Sigmaaldrich.com
03-312
12 reactions/Pk  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number03-312
Brand Family Chemicon®
Trade Name
  • Magna ChIRP
DescriptionMagna ChIRP Human HOTAIR lncRNA Probe Set
OverviewChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target for biotinylated antisense probes. The Magna ChIRP Human HOTAIR lncRNA Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of Human lncRNA HOTAIR. All probes are biotin-TEG-labeled at the 3-prime end. The oligonucleotides are split to two pools (even and odd) and are supplied ready for use in the ChIRP method.
Background InformationPervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP™ RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.
References
Product Information
Presentation10 mM Tris-HCl, 1mM EDTA, pH 8.0
Quality LevelMQ100
Applications
ApplicationThe Magna ChIRP Human HOTAIR lncRNA Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of Human lncRNA HOTAIR.
Key Applications
  • DNA-binding Proteins
  • DNA Sequencing
  • Protein Determination
  • Protein Interaction Assays
  • Purification with magnetic beads
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceVerified by Electrospray Ionization Mass Spectrometry. Final yields are determined using UV absorbance at OD260. Concentrations are determined using UV absorbance at OD260 before pooling. Oligos will manufactured under ISO 9001 conditions.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt store at -20°C. Avoid multiple freeze thaws. With proper storage and handling this product is stable for 6 months from the date of receipt.
Packaging Information
Material Size12 reactions/Pk
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-312 04054839317927

Related Products & Applications