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FCMAB250A4 Milli-Mark Anti-Bcl-2 Antibody, Alexa Fluor®488, clone AW604

This Milli-Mark Bcl-2 antibody is validated for use in Flow for the detection of the Milli-Mark Bcl-2 protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BCL2, Bcl-2 UniProt Number: P10415
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FCMAB250A4
100 tests  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberFCMAB250A4
Trade Name
  • Milli-Mark
DescriptionMilli-Mark Anti-Bcl-2 Antibody, Alexa Fluor®488, clone AW604
Background InformationBcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Bcl-2 also regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspases. It appears to Inhibit caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Further understanding in how Bcl-2 functions and interacts with other proteins to promote or inhibit apoptosis may lead to new treatments for cancer, neurological disorders and autoimmune diseases.
References
Product Information
FormatAlexaFluor®488
Control
  • Jurkat Cells
PresentationPurified mouse monoclonal IgG2a conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationThis Milli-Mark Bcl-2 antibody is validated for use in Flow for the detection of the Milli-Mark Bcl-2 protein.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to human Bcl-2
CloneAW604
HostMouse
SpecificityAntibody recognizes Bcl-2.
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Gene Symbol
  • BCL2
  • Bcl-2
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

SIZE: 239 amino acids; 26266 Da

SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.

SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

TISSUE SPECIFICITY: Expressed in a variety of tissues.

DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.

PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.

DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

SIMILARITY: Belongs to the Bcl-2 family
Molecular Weight26 kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaulated by flow cytometery using Jurkat cells.
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
FCMAB250A4 04053252290091

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies