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FCMAB288A6 Milli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3

This Milli-Mark Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 is validated for use in FC for the detection of Ubiquitin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism UniProt Number: P0CG48
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FCMAB288A6
100 tests  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCRbAlexaFluor®647Monoclonal Antibody
Description
Catalogue NumberFCMAB288A6
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3
Background InformationPolyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
References
Product Information
FormatAlexaFluor®647
HS Code3002 15 90
Control
  • HeLa cells
PresentationPurified recombinant rabbit monoclonal IgG1 conjugated to Alexa Fluor 647 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationThis Milli-Mark Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 is validated for use in FC for the detection of Ubiquitin.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen used was di-ubiquitin linked at Lys63.
EpitopeLys63 ubiquitination site
CloneApu3
HostRabbit
SpecificityRecognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21


SUBCELLULAR LOCATION: Cytoplasm. Nucleus.


PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.


MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).


SIMILARITY: Belongs to the ubiquitin family.
Molecular WeightMolecular weight varies.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using HeLa cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
FCMAB288A6 04053252680915

Documentation

Milli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark™ Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor®647, clone Apu3 Certificates of Analysis

TitleLot Number
Milli-Mark Anti-Ubiquitin, Lys63- Specific-Alexa Fluor#174;647, clone Apu3 - NRG1817667 NRG1817667
Milli-Mark™ Anti-Ubiquitin, Lys63- -2780979 2780979
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 - 2495478 2495478
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 - 2543761 2543761
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 - 3764825 3764825
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 -2650817 2650817
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 Conjugate 2951435
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3 Conjugate 3041058
Milli-Mark™ Anti-Ubiquitin, Lys63- Specific-Alexa Fluor®647, clone Apu3 - 2204968 2204968
Milli-Mark™ Anti-Ubiquitin, Lys63- Specific-Alexa Fluor®647, clone Apu3 - 2283911 2283911
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