Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

PIHA03050 Millicell Cell Culture Insert, 30 mm, HA mixed cellulose esters, 0.45 µm

Mixed Cellulose Esters insert with pore size of 0.45 µm used in a 6-well plate for in vitro Toxicology Assays, Cell Attachment, cell culture, Cell Differentiation, Cell Surface Receptor Assays, Microbial Attachment Assays & Polarized Uptake Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
PIHA03050
50  Individually blister packaged
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberPIHA03050
Trade Name
  • Millicell
DescriptionMillicell Cell Culture Insert, 30 mm, HA mixed cellulose esters, 0.45 µm
Background InformationWith Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.

Millicell inserts are available for 24-, 12-, or 6-well plates. The inserts are easily prepared for SEM and TEM visualizing techniques, and they are compatible with cellular and/or fluorescent stains.

Insert Formats:
Millicell Hanging Inserts: Preloaded in receivers or available standalone
- For co-culturing and permeability assays
- Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
- Available in 5 pore sizes and 3 diameters, including a 1 µm pore size that is optically transparent for better visualization by microscopy
- NEW: Available preloaded in 24-well receiver plates or as individual inserts in 3 diameters


Millicell Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies
- Available with Biopore (PTFE) membrane, MF-Millipore (mixed cellulose esters) membrane, and polycarbonate membrane

Millicell Organotypic Insert:
- For high cell viability and superior study of three dimensional explant structure
- Lower height allows them to fit inside a standard petri dish
- The Biopore™ (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
- The membrane is optically clear and optimized for long-term organotypic explant maintenance

Membrane Types:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

MF-Millipore™ Membrane (mixed cellulose esters)
- For exceptional anatomical and functional polarization

Isopore™ Membrane (polycarbonate)
- For growth of attachment-dependent cells without matrix

PET Membrane (polyethylene terephthalate)
- For growth of attachment-dependent cells without matrix

With Millicell® inserts, adherent or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Applications

Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry
References
Product Information
Device Configuration6-well plate
Filter CodeHA
Maximum Operating Temperature50 °C
Number of Wells1
Quality LevelMQ400
Applications
ApplicationMixed Cellulose Esters insert with pore size of 0.45 µm used in a 6-well plate for in vitro Toxicology Assays, Cell Attachment, cell culture, Cell Differentiation, Cell Surface Receptor Assays, Microbial Attachment Assays & Polarized Uptake Assays.
Key Applications
  • in vitro Toxicology Assays
  • Cell Attachment
  • Cell Culture
  • Cell Differentiation
  • Cell Surface Receptor Assays
  • Microbial Attachment Assays
  • Polarized Uptake Assays
Biological Information
MediaMF-Millipore
SterilitySterile
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Cardiac Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Pore Size0.45 µm
Dimensions
Height13 mm
Diameter3.15 cm
Filtration Area4.2 cm²
Filter Diameter (⌀)30 mm
Materials Information
Chemistry
  • Mixed Cellulose Esters (MCE)
Device Material
  • Polystyrene
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size50
Material PackageIndividually blister packaged
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PIHA03050 04053252664991