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411423 Mouse Anti-Human IgA, Secretory (HP6141)

This Mouse Anti-Human IgA, Secretory (HP6141) is validated for use in ELISA for the detection of Human IgA, Secretory.

Mouse Anti-Human IgA, Secretory (HP6141) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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411423
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
411423-400UG Plastic ampoule 400 μg
Description
Catalogue Number411423
Brand Family Calbiochem®
References
ReferencesHamilton, R.G., 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Reimer, C.B., et al. 1989. Immunol. Letters 21, 209.
Product Information
FormLiquid
FormulationIn PBS, pH 7.4.
PreservativeNone
Quality LevelMQ200
Applications
Application ReferencesELISA Hamilton, R.G., 1987. Clin. Chem. 33, 1707.
Key Applications Enzyme-Linked Immunosorbent Assay
Application NotesELISA (see comments and application references)
Application CommentsCan be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG: <0.01%
Human IgE: <0.01%
Human IgM: <0.01%
Human IgA1: <0.01%
Human IgA2: <0.01%
Human secretory IgA: 100%
Biological Information
ImmunogenHuman
CloneHP6141
HostMouse
IsotypeIgG₁
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
411423-400UG 04055977211191

Documentation

Mouse Anti-Human IgA, Secretory (HP6141) SDS

Title

Safety Data Sheet (SDS) 

Mouse Anti-Human IgA, Secretory (HP6141) Certificates of Analysis

TitleLot Number
411423

References

Reference overview
Hamilton, R.G., 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Reimer, C.B., et al. 1989. Immunol. Letters 21, 209.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2007 RFH
ApplicationELISA (see comments and application references)
DescriptionPurified mouse monoclonal IgG. Recognizes human IgA secretory subunit.
BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, CALBIOCHEM® technical monograph, The Human IgG Subclasses, is available free of charge.
HostMouse
Immunogen speciesHuman
CloneHP6141
IsotypeIgG₁
FormLiquid
FormulationIn PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsCan be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG: <0.01%
Human IgE: <0.01%
Human IgM: <0.01%
Human IgA1: <0.01%
Human IgA2: <0.01%
Human secretory IgA: 100%
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHamilton, R.G., 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Reimer, C.B., et al. 1989. Immunol. Letters 21, 209.
Application referencesELISA Hamilton, R.G., 1987. Clin. Chem. 33, 1707.