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69831 Mouse Ig-Primer Set

Mouse Ig-Primer Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69831
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
69831-3 Glass bottle 1 set
Description
OverviewThe Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.

In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.

Please call Technical Service if you need more information regarding individual Ig-Primer Sets.
Catalogue Number69831
Brand Family Novagen®
References
ReferencesLarrick, J.W., et al. 1989. Biochem. Biophys. Res.
Comm. 160, 1250. Jones, S.T. and Bendig, M.M. 1991.
Biotechnology 9, 88.
Product Information
•500 pmolMu IgM VH 3ʹ-1 Primer
•500 pmolMu IgG VH 3ʹ-2 Primer
•500 pmolMu Ig VH 5ʹ-A Primer
•500 pmolMu Ig VH 5ʹ-B Primer
•100 pmolMu Ig VH 5ʹ-C Primer
•100 pmolMu Ig VH 5ʹ-D Primer
•100 pmolMu Ig VH 5ʹ-E Primer
•100 pmolMu Ig VH 5ʹ-F Primer
•500 pmolMu Igκ VL 3ʹ-1 Primer
•500 pmolMu Igκ VL 5ʹ-A Primer
•500 pmolMu Igκ VL 5ʹ-B Primer
•100 pmolMu Igκ VL 5ʹ-C Primer
•100 pmolMu Igκ VL 5ʹ-D Primer
•100 pmolMu Igκ VL 5ʹ-E Primer
•100 pmolMu Igκ VL 5ʹ-F Primer
•100 pmolMu Igκ VL 5ʹ-G Primer
•500 pmolMu Igλ VL 3ʹ-1 Primer
•500 pmolMu IgMλ VL 5ʹ-A Primer
•10 µlMouse Positive Control PCR Template
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
69831-3 04055977274011

Documentation

Mouse Ig-Primer Set Certificates of Analysis

TitleLot Number
69831

References

Reference overview
Larrick, J.W., et al. 1989. Biochem. Biophys. Res.
Comm. 160, 1250. Jones, S.T. and Bendig, M.M. 1991.
Biotechnology 9, 88.

Citations

Title
  • Emi Hifumi, et al. (2008) Catalytic features and eradication ability of antibody light-chain UA15-L against Helicobacter pylori. Journal of Biological Chemistry 283, 899-907.
  • Anna S. Gardberg, et al. (2007) Molecular basis for passive immunotherapy of Alzheimer's disease. Procedings of the National Academy of Science 104, 15659-15664.
  • Kazuyo Toyoda, et al. (2007) Protein-bound 4-hydroxy-2-nonenal: an endogenous triggering antigen of anti-DNA response. Journal of Biological Chemistry 282, 25769-25778.
  • Manisha Goel, et al. (2004) Plasticity within the antigen-combining site may manifest as molecular mimicry in the humoral immune response. Journal of Immunology 173, 7358-7367.
  • Eric A. Grovender, et al. (2004) Single-chain antibody fragment-based Adsorbent for the extracorporeal removal of β2-microglobulin. Kidney International 65, 310-322.
  • Michael G. Rosenblum, et al. (2003) Design, expression, purification, and characterization, in vitro and in vivo, of an antimelanoma single-chain Fv antibody fused to the toxin gelonin. Cancer Research 63, 3995-4002.
  • Lingxun Duan, et al. (1994) Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody. Procedings of the National Academy of Science 91, 5075-5079.
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    User Protocols

    Title
    TB326 IgG Primer Sets