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480401 NFAT Inhibitor, Cell-Permeable - Calbiochem

The NFAT Inhibitor, Cell-Permeable controls the biological activity of NFAT. This small molecule/inhibitor is primarily used for Inflammation/Immunology applications.

NFAT Inhibitor, Cell-Permeable - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 11R-VIVIT, Nuclear Factor of Activated T cells Inhibitor, RRRRRRRRRRR-GGG-MAGPHPVIVITGPHEE

Primary Target: NFAT
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480401
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₄₇H₂₅₉N₆₇O₃₆S

Products

Catalog NumberPackaging Qty/Pack
480401-1MG Plastic ampoule 1 mg
Description
OverviewA cell-permeable version of NFAT Inhibitor (Cat. No. 480402) that is modified at the N-terminal with an eleven arginine transduction domain and a three glycine linker sequence. Prevents the activation and proliferation of T cells both in vitro ( ~ 43% at 1 µM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice). Shown to be effective in providing immunosuppression in a murine islet xenograft model. Unlike Cyclosporin A (Cat. No. 239835) and FK506, 11R-VIVIT does not cause side effects outside the immune system as a result of calcineurin (Cat. No. 539568) inhibition and therefore serves as a less toxic alternative for in vivo applications. The functional half-life of 11R-VIVIT has been estimated to be ~30 h in Jurkat cells.
Catalogue Number480401
Brand Family Calbiochem®
Synonyms11R-VIVIT, Nuclear Factor of Activated T cells Inhibitor, RRRRRRRRRRR-GGG-MAGPHPVIVITGPHEE
References
ReferencesNoguchi, H., et al. 2004. Nat. Med. 10, 305.
Product Information
ATP CompetitiveN
FormLyophilized white solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₄₇H₂₅₉N₆₇O₃₆S
Chemical formulaC₁₄₇H₂₅₉N₆₇O₃₆S
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetNFAT
Primary Target IC<sub>50</sub>Use at 1 µM for preventing the activation and proliferation of T cells
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceH-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Gly-Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Harmful
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
480401-1MG 07790788050641

Documentation

NFAT Inhibitor, Cell-Permeable - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

NFAT Inhibitor, Cell-Permeable - Calbiochem Certificates of Analysis

TitleLot Number
480401

References

Reference overview
Noguchi, H., et al. 2004. Nat. Med. 10, 305.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-August-2008 RFH
Synonyms11R-VIVIT, Nuclear Factor of Activated T cells Inhibitor, RRRRRRRRRRR-GGG-MAGPHPVIVITGPHEE
DescriptionA cell-permeable version of NFAT Inhibitor (Cat. No. 480402) that is modified at the C-terminus with an 11-arginine transduction domain and a 3-glycine linker sequence. Prevents the activation and proliferation of T cells both in vitro (~43% at 1 µM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice). Show to be effective in providing immunosuppression in a murine islet xenograft model. Unlike Cyclosporin A (Cat. No. 239835) and FK-506, 11R-VIVIT does not cause side effects outside the immune system as a result of calcineurin (Cat. No. 539568) inhibition, therefore serves as a less toxic alternative for animal studies. The functional half-life of 11R-VIVIT has been estimated to be ~30 h in Jurkat cells.
FormLyophilized white solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₄₇H₂₅₉N₆₇O₃₆S
Peptide SequenceH-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Gly-Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu-OH
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
ReferencesNoguchi, H., et al. 2004. Nat. Med. 10, 305.