Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

AG231 Neurofilament 70 kDa, porcine

Neurofilament 70 kDa, porcine MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Positive Control UniProt Number: P02547 Entrez Gene Number: 100521224
View Products on Sigmaaldrich.com
AG231
100 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberAG231
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionNeurofilament 70 kDa, porcine
OverviewPurified porcine axonal neurofilament 70 (low molecular weight, NF-L).
References
Product Information
PresentationLiquid in 6M Urea, 10 mM Phosphate, pH 7.5.
Quality LevelMQ100
Applications
Key Applications
  • Positive Control
Application NotesWestern blotting

Protein standard

ELISA

Radioimmunoassay

Optimal working dilution must be determined by the end user.
Biological Information
Concentration1 mg/mL
PurityContains approximately 90% NF-L and a small amount of NF-M.
Entrez Gene Number
Gene Symbol
  • NEFL
  • CMT1F
  • NF-L
  • NF68
  • CMT2E
  • NFL
UniProt Number
UniProt SummaryFUNCTION: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
SIZE: 549 amino acids; 62072 Da
DOMAIN: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
PTM: O-glycosylated (By similarity).
DISEASE: Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). & Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
SIMILARITY: Belongs to the intermediate filament family.
MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AG231 04053252406232

Documentation

Neurofilament 70 kDa, porcine SDS

Title

Safety Data Sheet (SDS) 

Neurofilament 70 kDa, porcine Certificates of Analysis

TitleLot Number
NEUROFILAMENT 70, PORCINE PURIFIED PROTEIN - 2436306 2436306
NEUROFILAMENT 70, PORCINE - 3145029 3145029
NEUROFILAMENT 70, PORCINE - 3587163 3587163
NEUROFILAMENT 70, PORCINE - 3891418 3891418
NEUROFILAMENT 70, PORCINE - 4034706 4034706
NEUROFILAMENT 70, PORCINE - 4132469 4132469
NEUROFILAMENT 70, PORCINE -2766392 2766392
NEUROFILAMENT 70, PORCINE -2822807 2822807
NEUROFILAMENT 70, PORCINE PURIFIED PROTEIN 3047042
NEUROFILAMENT 70, PORCINE PURIFIED PROTEIN - 1921340 1921340
Show more

Data Sheet

Title
NEUROFILAMENT 70, PORCINE PURIFIED PROTEIN